补肾阴对胚胎干细胞活动的影响  

Effects of Tonifying Kidney-Yin on the Activities of Embryonic Stem Cell

作　　者：安红梅[1] 胡兵[1] 史云峰[1] 许丽雯[1] 史秀峰[1] 陈方敏[2] 顾耘[1] 潘露茜[1] 王志[2] 

机构地区：[1]上海中医药大学附属龙华医院,上海200032 [2]上海中医药大学,上海201203

出　　处：《中国中医药信息杂志》2007年第6期24-26,共3页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：国家自然基金资助(30400574)

摘　　要：目的观察补肾阴中药复方对胚胎干细胞活动的影响。方法选用胚胎干细胞模型CRL-1825细胞株,加入补肾阴中药复方大鼠含药血清,观察补肾阴中药对干细胞分化、凋亡、细胞周期及其关键基因表达的影响。结果补肾阴中药复方含药血清可以抑制干细胞的分化和凋亡,促进干细胞增殖以及细胞周期的进程,促进干细胞Wnt、Oct4等基因的表达,下调P16INK4a基因的表达。结论补肾阴中药复方可以促进干细胞增殖、维持其不分化状态。Objective To study the effects of tonifying kidney-yin on activities of embryo stem cell. Methods Embryonic stem cell line CRL- 1825 was choosed as model cell in present study, and tonifying kidney-yin herb serum was added to CRL-1825 cell. The influence of tonifying kidney-yin on stem cell differentiation, cell cycle, apotosis and express of key genes were observed. R, amltl Tonifying kidney-yin can inhibit stem cell differentiation and apotosis, promote stem cell proliferation and progression of cell cycle. In addition, tonifying kidney-yin can up-regulate Wnt, Oct4 gene expression, and down-regulation P16^INK4a expression. Conclusions Tonifying kidney-yin can promote stem cell proliferation and maintain the state of stem cell.

关 键 词：补肾阴 中药 胚胎干细胞 

分 类 号：R289.11[医药卫生—方剂学]