金银花提取物体外抗甲型流感病毒FM1株的研究  

Inhibitory Effect of Extracts from Flos Lonicerae on Influenza A Virus FM1 Strain in Vitro

作　　者：潘曌曌[1] 王雪峰[2] 闫丽娟[2] 南春红[2] 岳志军[2] 

机构地区：[1]辽宁中医药大学,辽宁沈阳110032 [2]辽宁中医药大学附属医院,辽宁沈阳110032

出　　处：《中国中医药信息杂志》2007年第6期37-38,51,共3页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：国家教育部博士点基金课题(20040162004)

摘　　要：目的观察金银花提取物体外抑制甲型流感病毒FM1株的作用。方法采用血凝试验方法,测定不同浓度金银花提取物体外和鸡胚内抑制流感病毒效价,分析金银花提取物对病毒增殖的影响。结果与病毒对照组比较,金银花提取物组血凝效价降低,其抑制作用时间可从1h持续到24h,其对病毒的抑制作用随着药物浓度的降低而逐渐减弱;金银花提取物在100、50、25mg/mL时,对感染流感病毒鸡胚具有预防和保护治疗作用(P<0.001),而在100mg/mL时的预防作用明显优于阳性对照病毒唑组(P<0.05)。结论金银花提取物可有效抑制甲型流感病毒FM1株。Objective To observe the effect of extracts from Flos Lonicerae inhibiting influenza A virus FM1 strain. Metlhod Use the method of hemagglutination test, determine the hemagglutination inhibition titer that extracts from Flos Lonicerae inhibiting influenza A virus in vitro and in embryonated egg. Result Compared with the control group, the hemagglutination inhibition titer of extracts from Flos Lonicerae group were degraded. The depressant effect kept on from 1 hour to 24 hour, and the depressant effect were degraded with the stepping down of the concentration. At the concentration of 100, 50, 25 mg/mL, extracts from Flos Lonicerae had the preventative effect and therapeutical effect on embryonated egg infected by influenza A virus （P〈0,001）. At the concentration of 100 mg/mL, the preventative effect of extracts from Flos Lonicerae group was better than Ribavirin group （P〈0.05）. Conclusion Extracts from Flos Lonicerae can suppress influenza A virus FM1 strain actively.

关 键 词：甲型流感病毒 金银花提取物 绿原酸 鸡胚 

分 类 号：R285.5[医药卫生—中药学]