Nav1.1抗体对豚鼠心室肌细胞钠电流的影响  

The effect of antibody of Nav1.1 on sodium current in guinea pig ventricular cells

作　　者：张涛[1] 程龙献[1] 雷鸣[1] 史钰芳[1] 刘坤[1] 高翔[1] 赵芳[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院心内科,湖北武汉430022

出　　处：《中国心脏起搏与心电生理杂志》2007年第3期248-250,共3页Chinese Journal of Cardiac Pacing and Electrophysiology

基　　金：国家自然科学基金资助项目(项目编号:30470634)

摘　　要：目的探讨Nav1.1抗体对豚鼠心室肌细胞钠电流(INa)的影响。方法采用全细胞膜片钳技术,观察急性分离的豚鼠心室肌细胞在应用Nav1.1抗体后心肌INa的变化。结果在Nav1.1抗体作用下,钠离子通道电流密度呈浓度依赖性减小。用药前与三个浓度抗体组(1∶100,1∶50,1∶25)的电流密度分别为34.22±6.22,32.13±3.45,25.49±7.4,19.23±1.69pA/pF。其中1∶25Nav1.1抗体明显降低钠电流密度(P<0.05),并且,1∶25Nav1.1抗体使INa电流-电压曲线明显向正电位方向移动,但是其稳态失活曲线和恢复曲线没有显著性改变。结论Nav1.1可能对INa有影响。Objective To study the effect of antibody of Navl. 1 on sodium current in guinea pig ventricular cells. Methods Detected the sodium current before and after applying antibody of Nav1. 1 （ 1 ： 100,1 ： 50,1 ： 25） to the isolated guinea pig ventrieular cells with whole cell patch clamp recording. Results Antibody of Navl. 1 decreased the voltage activated Na^＋ currents in a concentration dependent manner. The density of Na ^＋ current of control was 34.22 ± 6.22pA/ pF, while those of test were 32.13 ± 3.45pA/pF, 25.49 ± 7.4pA/pF, and 19.23 ±1.69pA/pF respectively. Sodium current was inhibited by 1 ： 25 anti-Nav1. 1 significantly. 1：25 anti-Nav1. 1 shifted Ⅰ-Ⅴ relationship curve to more positive potentials. However, anti-Nav1. 1 didn＇t change the steady-state recovery and inactivation of Na^＋ currents. Conclusions Sodium channel subtypes Nav1. 1 contributes to the sodium current in ventricular cells, [ Chinese Journal of Cardiac Pacing and Electrophysiology ,2007,21 （ 3 ） ：248 - 250 ]

关 键 词：电生理学 钠电流 心室肌细胞 抗体 

分 类 号：R331.38[医药卫生—人体生理学]