VEGF抗体对兔同种异体肌腱移植作用的实验研究  

An experimental study about effect of neutralizing antibody to VEGF on tendon allogeneil graft

作　　者：耿树岩[1] 王宏光[2] 张文强[3] 窦洪磊[1] 唐胜建[1] 

机构地区：[1]潍坊医学院整形外科研究所,山东潍坊261041 [2]寿光市人民医院创伤骨科,山东寿光262700 [3]千佛山医院创伤骨科,山东济南250014

出　　处：《实用手外科杂志》2007年第2期93-95,共3页Journal of Practical Hand Surgery

摘　　要：目的探讨VEGF抗体在异体肌腱移植愈合过程中的作用。方法将深低温冷冻的同种异体肌腱移植修复新西兰大白兔跟腱缺损,实验组腱周局部滴加VEGF多克隆抗体,对照组滴加生理盐水。术后1、2、4、8周四个时相点处死动物,标本进行大体观察、组织学观察、胶原含量测定及生物力学测试。结果实验组肌腱粘连等级评分较对照组明显改善,组织学观察可见术后早期实验组较对照组炎性细胞及新生毛细血管数量少,胶原含量差异不明显,但术后8周实验组胶原纤维排列更规则。生物力学测试显示实验组同对照组断裂载荷无显著差异。结论局部应用VEGF抗体,可以减轻移植肌腱粘连,而对移植肌腱的愈合无明显影响。Objective To investigate the effects of neutralizing antibody to VEGF in agglutination of tendon allogeneil graft. Methods Using profound hypothermia homogeneous variant tendon graft to repair the defects of New Zealand albino rabbit： the experiment group： around the tendon dropwise with polyclonal antibody of VEGF, the control group dropwise with Sodium Chloride. Put the animals to death in 1 week, 2 weeks, 4 weeks and 8 weeks separately postoperation. Samples were un- dertaken general observation, histological observation,collagen assaying and biomechanical test. Results Adhesion score of tendon in the experiment group is better than the control group.The phlegmasia cell population and newly born micrangium of the experiment group is less than the control group in the early postoperation. The collagen contents have no obviously discrepancy in two groups at the same time, but the arrangement of collagenous fibers of the experiment group is better＂ than the control group on postoperation 8 weeks. Biomechanical test shows there is no significant deviation in two groups in broken load. Conclusion Topical application of neutralizing antibody to VEGF on tendon allogeneil graft can lessen adhesion of tendon graft, but has httle effects on tendon heal.

关 键 词：血管内皮生长因子 同种异体肌腱移植 修复 

分 类 号：R622[医药卫生—整形外科]