脑摄取平衡时异丙酚在犬脑不同区域的分布  

Cerebral distribution of propofol at cerebral propofol uptake equilibrium in dogs

作　　者：林春水[1] 卢刚[1] 古妙宁[1] 刘长涛[1] 万山河[2] 徐伟[2] 

机构地区：[1]南方医科大学南方医院麻醉科,广东广州510515 [2]南方医科大学药学院,广东广州510515

出　　处：《南方医科大学学报》2007年第6期836-838,共3页Journal of Southern Medical University

基　　金：广东省科技计划项目(2004B30601006)~~

摘　　要：目的研究脑摄取达到平衡状态时异丙酚在犬脑组织的摄取和分布。方法6只犬各以异丙酚7mgkg静脉注射,续以70mgkg·h恒速静脉泵注。泵注30min(T30)、50min(T50)时分别取颈内动脉和颈内静脉血。T50时处死实验犬,解剖取额叶、顶叶、颞叶、海马、扣带回、丘脑、中脑、桥脑、小脑组织。采用高效液相色谱技术测量异丙酚血浆浓度和脑组织浓度。结果颈内动脉、静脉血浆异丙酚浓度(μgml)在T30时分别为3.107±1.067和3.095±1.085(t=0.671,P=0.532);T50时分别为3.091±1.101和3.117±1.091(t=1.890,P=0.117)。T50时,额叶、顶叶、颞叶、海马、扣带回、丘脑、中脑、桥脑、小脑异丙酚浓度(μgg)分别为3.085±1.123、3.116±1.125、3.073±1.159、3.117±1.090、3.075±1.178、3.073±1.146、3.075±1.151、3.102±1.174、3.072±1.192,各脑组织异丙酚浓度差异无统计学意义(F=0.127,P=0.998)。结论本实验条件下,犬脑对异丙酚的摄取在T50时处于平衡状态;摄取平衡时异丙酚在犬脑组织呈均衡分布。Objective To investigate the cerebral distribution ofpropofol during continued infusion at a constant rate when the cerebral propofol uptake reaches equilibrium in dogs, Methods Six healthy 1-year-old male dogs were used in this study. The venous channel was established in the great saphenous vein of the right posterior limb. Anesthesia was induced with a single bolus injection of propofol （7 mg/kg）, followed by propofol infusion at a constant rate of 70 mg/（kg·h） using a microinfusion pump. The blood samples were taken from the right internal carotid and internal jugular vein at 30 min （T30） and 50 min （T50） during propofol infusion for measurement of plasma propofol concentrations with high performance liquid chromatography （HPLC）. At T50, the fi-ontal lobe, parietal lobe, temporal lobe, hippocampus, cingulate gyrus, thalamus, midbrain, ports, and cerebellum were dissected respectively for determination of propofol concentrations. Results Propofol concentrations in the internal carotid artery and internal jugular vein blood plasma were 3.107± 1.067, 3.095 ± 1.085 μg/ml at T30 and 3.091±1.101, 3.117±1.091 μg/ml at T50, respectively, showing no significant differences （P〉0.05）. Propofol concentrations in the frontal lobe, parietal lobe, temporal lobe, hippocampus, cingulate gyrus, thalamus, midbrain, pons, cerebellum at T50 were 3.085±1.123; 3.116±1.125, 3.073±1.159, 3.117±1.090, 3.075±1.178, 3.073±1.146, 3.075±1.151, 3.102±1.174, and 3.072±1.192 μg/g respectively, suggesting homogeneous propofol distribution in these cerebral tissues （P〉 0.05）. Conclusions At T50, the cerebral uptake of propofol reached equilibrium when propofol is distributed homogeneously in the cerebral tissues in dogs.

关 键 词：异丙酚 静脉麻醉 脑 犬 

分 类 号：R614.24[医药卫生—麻醉学]