高效液相色谱法同时测定玉屏风汤剂中6种成分  

Simultaneous determination of six components in Yupingfeng Decoction by high-performance liquid chromatography

作　　者：游文玮[1] 吴昭晖[2] 邹敏[1] 谭晓梅[3] 

机构地区：[1]南方医科大学药学院,广东广州510515 [2]南方医科大学分析测试中心,广东广州510515 [3]南方医科大学中药新药重点实验室,广东广州510515

出　　处：《南方医科大学学报》2007年第6期884-886,共3页Journal of Southern Medical University

摘　　要：目的建立以高效液相色谱法同时测定玉屏风散中2类化合物(黄酮类和色原酮类)6种有效成分升麻苷、升麻素、5-O-甲基维斯阿米醇苷、槲皮素、亥茅酚苷、芒柄花素含量的分析方法。方法采用AgilentExtend-C18色谱柱(4.6mm×250mm,5μm),柱温25℃;流动相为甲醇-1%乙酸水溶液,梯度洗脱,流速0.8mlmin;检测波长254nm。结果升麻苷、升麻素、5-O-甲基维斯阿米醇苷、槲皮素、亥茅酚苷、芒柄花素分别在90～1810ng、97～1940ng、190～1906ng、105～3144ng、88～2625ng、109～3279ng范围内标准曲线呈良好的线性关系,相关系数均在0.9999以上;平均加样回收率分别为98.2%,99.1%,97.3%,97.8%,98.8%,99.2%。结论方法简便快速,结果准确,重复性好,线性范围宽,灵敏度高,可用于玉屏风制剂中升麻苷、升麻素、5-O-甲基维斯阿米醇苷、槲皮素、亥茅酚苷和芒柄花素含量的同时测定,为玉屏风散质量监控及新剂型研究提供了更加合理、可靠的检测方法。Objective To establish a HPLC-based method for simultaneous determination of 2 classes of compounds （flavonoids and chromones） and 6 their effective components, （including prin-O-glucosylcimifugin, cimifugin, 4＇-O-β-D-glucosyl- 5-O-methylvisamminol, quercetin, sec-o-glucosylhamaudol and formononetin）, in Yupingfeng Decoction. Methods HPLC-based separation of the agents was performed on Agilent Extend-C18 column （4.6 mm×250mm, 5 μm） at 25℃ with the mobile phase of MeOH-1% acetic acid water solution （gradient elution）, flow rate of 0.8 ml/min and detection wavelength of 254 nm. Results and Conclusion HPLC allowed simultaneous quantitative determination of the 6 components in Yupingfeng Decoction, and they showed good linear relationships when their sample amount ranged 90-1810 ng, 97-1940 ng, 190-1906 ng, 105-3144 ng, 88-2625 ng and 109-3279 ng, respectively, with correlation coefficients all beyond 0.9999 and average recovery rates of 98.2%, 99.1%, 97.3%, 97.8%, 98.8% and 99.2%, respectively. This simple and convenient method accommodated a broad linear range with high sensitivity and precise and reproducible results.

关 键 词：高效液相色谱法 玉屏风 升麻苷 升麻素 5-O-甲基维斯阿米醇苷 槲皮素 亥茅酚苷 芒柄花素 

分 类 号：R284[医药卫生—中药学]