转基因抗虫棉花木质素含量及其生物合成关键酶活性研究  

Lignin Content and Its Activity of Key Metabolic Enzymes in Transgenic Cotton

作　　者：孙彩霞[1] 陈振华[2] 缪璐[1] 牛翰杰[1] 

机构地区：[1]东北大学理学院,辽宁沈阳110004 [2]中国科学院沈阳应用生态研究所,辽宁沈阳110016

出　　处：《东北大学学报（自然科学版）》2007年第6期867-870,共4页Journal of Northeastern University(Natural Science)

基　　金：辽宁省科技攻关项目(2004201003);辽宁省博士启动基金资助项目(20041008);国家自然科学基金资助项目(40101016)

摘　　要：通过盆栽试验,以不同转基因抗虫棉花及其亲本对照为供试对象,研究木质素含量及其生物合成关键酶活性.结果表明:与非转基因棉花比较,在蕾期及铃期,转基因抗虫棉花Z30,CCRI41,SGK321叶片、茎秆组织中的木质素含量及叶片内的苯丙氨酸解氨酶和过氧化物酶活性发生了一些变化,但其显著性改变仅特异性地发生在某些品种、某些组织或某些时期内.在蕾期,转Bt基因棉花Z30茎秆组织中的木质素含量比其常规棉对照Z16显著下降,幅度达34.96%;转CpTI-Bt棉花CCRI41茎秆组织和SGK321叶片组织中的木质素含量比其常规棉对照显著下降,幅度分别为27.15%,13.22%.所有供试转基因抗虫棉花的木质素总量未见显著性改变.Taking different transgenic cotton as test materials for pot experiment, the lignin content, POD and PAL activities in different cotton tissues were studied to reveal the change of lignin metabolism in transgenic cotton. The results showed that in comparison with non-transgenic cotton, the lignin content, POD and PAL activities in transgenic cotton change somewhat in some varieties, stages and tissues. At squaring stage, the lignin content lowers significantly by 34.96%, 27.15% and 13.22% in Z30 stems, CCRI41 stems and SGK321 leaves, respectively. However, no significant change is found in the total lignin content for all transgenic cotton in experiments.

关 键 词：转基因棉花 木质素 酶活性 生物合成 

分 类 号：Q819[生物学—生物工程] Q945