抗痛风颗粒中青风藤TLC鉴别与青藤碱的含量测定  

TLC Identification of Caulis Sinomenii and the Content Determination of Sinomenine in Anti gout Granules

作　　者：陈勇[1] 邓家刚[1] 王勤[1] 刘婧[1] 潘丽娜[1] 丁光华[1] 

机构地区：[1]广西中医学院药学院,广西南宁530001

出　　处：《中国实验方剂学杂志》2007年第6期11-13,共3页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:建立抗痛风颗粒中青风藤的TLC定性鉴别方法和青藤碱的高效液相色谱含量测定方法。方法:以青藤碱为对照品,采用甲苯-乙酸乙酯-甲醇-氨水(2∶4∶2∶1)为展开剂,改良碘化铋钾溶液为显色剂,对抗痛风颗粒中青风藤进行薄层色谱法定性鉴别。采用Agilent extend C18柱(4.6 mm×150 mm,5μm),甲醇-0.1%磷酸溶液(用三乙胺调pH至10)(38∶62)为流动相,262 nm为检测波长,测定了抗痛风颗粒中青藤碱的含量。结果:抗痛风颗粒薄层色谱中,青藤碱斑点清晰,阴性对照无干扰。HPLC定量分析中,青藤碱线性范围为0.414～2.484μg(r=0.999 8),平均回收率为97.6%,RSD=0.32%。结论:所建立的分析方法简便可行,可用于该制剂的质量控制。Objective： To develop a TLC method for Caulis Sinomenii indentification and a HPLC method for Sinomenine determination in the anti gout granules. Methods： Caulis Sinomenii was identified on a silicagel thin layer plate dev reference eloped with methylbenzene ： acetidin： methanol ： ammonia water （2 ： 4 ： 2 ： 1 ） and with sinomenine as the chemical substance, improved. Dragendorff reagent was used for visualization. The separation of Sinomenium was carried out on column Extend C18 4.6 mm × 150 mm, 5 μm. The mobile phase was the combination of methanol and the solution of 0.1% phosphoric acid（38：62）, and the detection wareleng the was set at 262 nm. Result： Sinomenine spots on TLC plates was separated detection warelength well. The linear range is between 0.414 and 2.484 μg （ r = 0.999 8） and the recovery is 97.6% （RSD = 0.32% ）. Conclusions： The methods are sensitive, simple; accurate and can be used for the control of anti gout granules.

关 键 词：抗痛风颗粒 青风藤 青藤碱 薄层色谱法 高效液相色谱法 

分 类 号：R283.6[医药卫生—中药学]