补阳还五汤及其有效组分生物碱和苷对动脉血栓形成大鼠抗凝系统活性的影响  

Effect of Buyang Huanwu Decoction and Its Alkaloid and Glycoside on Anticoagulative System Arterial Thrombosis Rat

作　　者：唐映红[1] 梁燕[2] 张淑萍[2] 杨静[2] 邓冰湘[2] 邓常青[2] 

机构地区：[1]湖南中医药大学药学院药理教研室,湖南长沙410208 [2]湖南中医药大学病理生理实验室,湖南长沙410007

出　　处：《中国实验方剂学杂志》2007年第6期39-42,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：教育部科学技术研究重点项目(204100);湖南省教育厅重点项目(03A033)

摘　　要：目的:研究补阳还五汤及其生物碱、苷两类有效组分对FeCl3所致大鼠颈总动脉血栓形成后抗凝血酶Ⅲ(AT-Ⅲ)和蛋白C(PC)活性的影响。方法:大鼠给药后以35%FeCl3诱导颈总动脉血栓形成,以发色底物法测定血浆AT-Ⅲ和PC活性。结果:FeCl3可诱导颈总动脉血栓形成。补阳还五汤及其有效组分生物碱、苷可使颈总动脉血栓重量减轻(P<0.05)。模型组AT-Ⅲ活性低于假手术组(P<0.01);与模型组比较,生物碱组和补阳还五汤原方组AT-Ⅲ活性均显著降低(P<0.01),而苷组与模型组比较差异无显著性意义(P>0.05)。模型组PC活性与假手术组比较有所降低(0.10>P>0.05);与模型组比较,生物碱组PC活性显著降低(P<0.05),而苷组和原方组PC活性与模型组比较则显著性升高(P<0.01),且苷和原方组PC活性高于假手术组(P<0.01)。结论:补阳还五汤、生物碱和苷可抑制血栓形成。补阳还五汤原方既可能通过与AT-Ⅲ结合而发挥抗凝作用,又可防止血栓形成后血浆中PC的消耗。其有效组分生物碱既可能通过与AT-Ⅲ结合而灭活凝血因子,又可能促进了PC对凝血因子的灭活,从而发挥抗凝作用。苷的抗血栓可能不是通过AT-Ⅲ和PC介导的。Objective：To investigate effect of Buyang Huanwu Decoction （BHD） and its active fractions such as alkaloid and glycoside on activities of antithromhin Ⅲ （ AT- Ⅲ ） and protein C （ PC ） after carotid arterial thrombosis induced by ferric chloride in rats. Methods： Rats were administered with tested drugs and then left carotid artery was applied with FeCl3 to form thromhus. The activities of antithrombin Ⅲ （AT-Ⅲ ） and protein C（PC） in plasma in rats were determined by chromogenic suhstrate assay. Results： BHD, alkaloid and glycoside could decrease thrombotic weight （ P 〈 0.05）. The activities of AT-Ⅲ in the model group were significantly lower than those of the sham-operated group（ P 〈 0.01）. The activities of AT-Ⅲ in BHD and alkaloid group were markedly lower than those of the model group（ P〈 0.01 ）, No significant difference was found in activities of AT-Ⅲ between the glycoside and model group （P 〉 0.05 ）. The activities of PC in the model group were slightly lower than those of the sham-operated group （0.10 〉 P 〉 0.05）.Compared with the model group, the activities of PC in alkaloid group were decreased （ P 〈 0.05）. But the activities of PC in BHD and glycoside group were increased when compared to the model group（ P 〈 0.01 ） and sham group（ P 〈 0.01）. Conclusions： BHD, alkaloid and glycoside could inhibit thrombosis. Antithrombotic actions of BHD may be related to binding with AT-Ⅲ, and it could also prevent the consumption of PC after carotid arterial thrombosis. Antithrombotic action of alkaloid may concerne inactivating coagulation factors by binding with AT-Ⅲ or accelerating inactivation of coagulation factors by PC.Antithrombotic action of glycoside may not be related to AT-Ⅲ and PC.

关 键 词：补阳还五汤 动脉血栓形成 抗凝血酶Ⅲ 蛋白C 

分 类 号：R285.5[医药卫生—中药学]