低剂量X射线在优化pEgr-IL18-B7.1基因-放疗方案中的作用  

Effect of low dose whole-body X-irradiation on the efficacy of pEgr-IL18-B7.1 gene-radiotherapy

作　　者：武宁[1] 陈光伟[2] 刘永哲[1] 金顺子[1] 刘树铮[1] 

机构地区：[1]吉林大学公共卫生学院卫生部放射生物学重点实验室,长春130021 [2]吉林省卫生厅卫生监督所

出　　处：《中华放射医学与防护杂志》2007年第3期223-225,共3页Chinese Journal of Radiological Medicine and Protection

基　　金：国家自然科学基金资助项目（30100033）

摘　　要：目的观察低剂量X射线全身照射在接种Lewis肺癌小鼠的pEgr-IL18-B7.1基因-放疗中的抑瘤作用。方法基因-放疗组中小鼠肿瘤局部注射由多聚乙烯亚胺包裹的pEgr-IL18-B7.1重组质粒,分别接受由2GyX射线局部照射和0.075GyX射线全身照射组合的不同治疗方案,观察各种治疗方案在荷瘤小鼠中的抑瘤作用。结果与单纯多次大剂量X射线局部照射相比,单次大剂量X射线局部照射后加多次低剂量全身照射在联合pEgr-IL18-B7.1基因治疗中能体现出更为有效的抑制肿瘤生长作用。结论低剂量X射线全身照射作为一种优化手段,可有效增强pEgr-IL18-B7.1基因-放疗的抑瘤效果。Objective To observe the therapeutic effect of whole-body irradiation with low dose X-rays in mice bearing Lewis lung carcinoma under recombinant plasmid pEgr-IL18-B7. 1 gene-radiotherapy. Methods The pEgr-IL18-B7.1 recombinant plasmids mediated by polyethylenimine were injected locally into tumors of the mice with gene-radiotherapy ,and then the tumors received different therapeutic regimens containing local X- irradiation with 2 Gy and whole-body X-irradiation with 0.075 Gy, respectively. The anti-tumor effects of low dose X-rays in optimizing the protocol of pEgr-IL18-B7.1 gene-radiotherapy on the tumor-bearing mice were observed. Results As compared with repeated high dose local X-irradiation alone, single high dose local X- irradiation in combination with repeated low dose of whole-body X-irradiation showed more significant inhibition of tumor growth under pEgr-IL18-B7.1 gene-radiotherapy. Conclusions Low dose whole-body X-irradiation superimposed upon a local high dose could significantly enhance the anti-tumor effect in the protocol of pEgr- IL18-B7.1 gene-radiotherapy.

关 键 词：低剂量X射线 pEgr-IL18-B7.1重组质粒 基因-放疗 LEWIS肺癌细胞 

分 类 号：R686[医药卫生—骨科学]