^125I粒子持续低剂量率照射对人前列腺癌细胞的抑制作用  

The inhabitation effect of ^125 I seed irradiation on prostate cancer cell

作　　者：廖安燕[1] 王俊杰[1] 赵勇[2] 王济东[1] 庄洪卿[1] 

机构地区：[1]北京大学第三医院肿瘤治疗中心,100083 [2]中国科学院动物研究所生物膜国家重点实验室

出　　处：《中华放射医学与防护杂志》2007年第3期226-228,共3页Chinese Journal of Radiological Medicine and Protection

基　　金：国家自然科学基金资助项目（30572137）

摘　　要：目的探讨^125I粒子持续低剂量率照射对人前列腺癌细胞株（PC3）增殖抑制以及对细胞周期的影响。方法^125I粒子（初始剂量率2.77cGy/h）和^60Coγ射线（吸收剂量率2.215Gy/min）对体外培养的PC3细胞进行0、0.5、1、1.5、2、4、6和8Gy照射,用细胞计数、锥虫蓝染色和集落形成法检测细胞增殖、细胞活力和细胞存活率的情况;用单击多靶模型拟合剂量存活曲线;用流式细胞仪检测细胞周期。结果随着照射剂量的增加,^125I粒子照射组的细胞生长比^60Coγ射线组更加明显地受到抑制（P〈0.05）。^125I粒子照射PC3细胞D0值为2.243,Dq为0.87,N为1.618,SF2为0.5。^60Co照射组PC3细胞放射生物学参数D0值为2.824,Dq为1.08,N为1.587,SF2为0.7。^60Co和125I粒子的RBE比值为1.4。与空白对照组相比,^125I粒子组和^60Co组4Gy照射细胞24h后均出现G2期阻滞。结论^125I粒子照射能抑制人前列腺癌细胞的增殖并能使PC3细胞阻滞于G2期。Objective To study the effect on prostate cancer cell proliferation and cell cycle treated by continuous low dose rate ^125I irradiation. Methods PC3 cell was irradiated with ^125I （2.77 cGy/h） and ^60Co （2.215 Gy/min） at various doses. The cellular proliferation, vitality and cell surviving rate were evaluated by cytometric methods, typan-blue stain and the colony-forming assay after radiation. The cell cycle distribution was investigated by FCM. Results The death rate of PC3 cell exposed to ^125I was higher than that exposed to ^60 Co gamma rays with the increase of the irradiation dose. The parameters of radiobiology of PC3 irradiated by ^125I seeds were： Do = 2.243, D q = 0.87, N = 1.618, SF2 = 0. 5 whereas the parameters of ^60 Co were ： Do = 2. 824, D q = 1.08, N = 1.587, SF2 = 0.7.The RBE for ^125I compared with ^60Co gamma rays is 1.4.The cell cycle distribution after 4 Gy ^125I or ^60 Co irradiation was similar, with increasing in the proportion of cells in G2 phase after 24 h. Conclusions ^125I irradiation could inhibit the proliferation of PC3 and induced G2 phase arrest.

关 键 词：^125I放射性粒子 前列腺癌细胞 细胞增殖 细胞周期 

分 类 号：R686[医药卫生—骨科学]