多房棘球绦虫混合重组BCG-EmⅡ/3和BCG-Em14-3-3疫苗诱导小鼠的免疫保护力观察  

Protective effect of immunization with mixed recombinant BCG-EmⅡ/3 and BCG-Em14-3-3 vaccine against Echinococcus multilocularis in mice

作　　者：李文桂[1] 王鸿[1] 朱佑明[1] 

机构地区：[1]重庆医科大学附属第一医院传染病寄生虫病研究所,重庆400016

出　　处：《第三军医大学学报》2007年第13期1276-1279,共4页Journal of Third Military Medical University

基　　金：国家自然科学基金(30500423);教育部重点项目(205131)~~

摘　　要：目的探讨多房棘球绦虫混合重组BCG-EmⅡ/3和BCG-Em14-3-3疫苗免疫鼠后对Em原头节攻击感染的保护性作用。方法将混合重组BCG疫苗采用皮下注射和鼻腔内接种分别免疫BALB/c小鼠,免疫后8周用多房棘球绦虫原头节进行攻击感染,感染后18周剖杀小鼠,计算减蚴率,测定血清中IgG及其亚类和IgE水平;分离脾细胞,流式细胞仪检测脾CD4+和CD8+T淋巴细胞亚群的百分比,同时设有空载体、BCG和PBS对照。结果疫苗接种组的减蚴率为45·29%～76.47%,血清IgG、IgG2a、IgG2b和IgE水平明显升高,IgG1和IgG3显著降低;疫苗接种组的脾CD4+T细胞亚群显著增加,CD8+T细胞亚群无明显变化,CD4+/CD8+亚群比值明显升高。结论多房棘球绦虫混合重组BCG-EmⅡ/3和BCG-Em14-3-3疫苗鼻腔内接种是一种较好的途径,IgG、IgG2a、IgG2b和IgE以及CD4+T细胞亚群在疫苗诱导的保护力中起重要作用。Objective To investigate the protection by immunization with mix recombinant BCG-Em Ⅱ/3 and BCG-Em14-3-3 vaccine of Echinococcus multilocularis （Era） and against challenge of Em protoscolexes. Methods BALB/C mice were subcutaneously or intranasally vaccinated with mixed recombinant BCG vaccines respectively. Eight weeks later, the immunized mice were challenged with Em protoscolexes. On the 18^th week after vaccination, the mice were killed to count the rate of reduced alveolar echinococcus weight and to examine the levels of IgG and its subclasses, IgE; the spleen cells were separated to measure subsets of CD4^＋ and CD8^＋ T cells by FACsort. Blank vector, BCG and PBS served as control. Results In the immunized groups, the rate of reduced alveolar echinococcus weight was 45.29% - 76.47% ; the levels of IgG, IgG2a, IgG2b and IgE increased apparently while IgG1 and IgG3 decreased remarkably; CD4^＋ subset and the ratio of CD4^＋/ CD8^＋ increased obviously, but CD8^＋ subset had no change. Conclusion Intranasal vaccination with mix recombinant BCG-Em Ⅱ/3 and BCG-Em14-3-3 vaccine is a good way for immunization. IgG, IgG2a, IgG2b, IgE and CD4^＋ T cell subset may play an important role in protecting against challenge of Em protoscolexes.

关 键 词：多房棘球绦虫 重组BCG-EmⅡ/3疫苗 重组BCG-EM14-3-3疫苗 保护力 

分 类 号：R383.3[医药卫生—医学寄生虫学] R392.11[医药卫生—基础医学]