含去痴灵挥发油的大鼠血清对体外培养新生小鼠皮层神经元的营养作用  

Neurotrophic effect of the Quchiling volatile oil-containing rat serum on the cultured newborn mouse cortical neurons

作　　者：刘美英[1] 肖飞[1] 李晓光[1] 罗焕敏[1] 

机构地区：[1]暨南大学药学院神经药理研究室,广州510632

出　　处：《中国新药杂志》2007年第11期849-852,共4页Chinese Journal of New Drugs

基　　金：广东省科技计划项目(2004B30101012);广州市科技计划项目(2005Z3-E5081)

摘　　要：目的:研究含去痴灵挥发油的大鼠血清对体外培养新生小鼠皮层神经元的营养作用。方法:采用血清药理学方法,制备含去痴灵挥发油的大鼠血清。体外培养新生小鼠皮层神经元,在无血清培养液中加入含去痴灵挥发油的大鼠血清,使培养基中含药血清的最终体积分数分别为0.1%,0.5%和2.5%,设立空白血清对照组和重组人碱性成纤维细胞生长因子(rhbFGF,终浓度为20 ng.mL-1)阳性对照组。观察培养48 h细胞的形态学改变,定量比较神经元突起数目和长度;用四甲基偶氮唑蓝(MTT)比色法检测培养d 4和d 8神经元存活数;并用RT-PCR测定培养d 4时神经元细胞的微管相关蛋白2(MAP2)的mRNA含量。结果:0.5%和2.5%的含去痴灵挥发油的大鼠血清均能使神经元突起数目和长度增加、不同培养期细胞存活数增加、MAP2基因表达量增加。结论:含去痴灵挥发油的大鼠血清对新生小鼠皮层神经元具有营养作用。Objective： To study the neurotrophic activity of the Quchiling volatile oil-containing rat serum on the cultured newborn mouse cortical neurons. Methods： The neurons were cultured with 0.1% ,0.5% and 2.5% Quchiling volatile oil-containing rat serum, blank serum control and rhbFGF （positive control）. The number and length of neurites,and the survival neurons count were analyzed by microscopy,the effect of the Quchiling volatile oil-containing rat serum on the viability of neurons was evaluated by MTT assay in different culture time, and the level of MAP2 mRNA was determined by RTPCR. Results： Quchiling volatile oil-containing rat serum （0.5% , 2.5% ）increased the numbers and length of neurites, the number of survival neurons and the level of MAP2 mRNA 72h after treatment with the Quchiling volatile oil-containing rat serum. Conclusions： Quchiling volatile oil-containing rat serum has a neurotrophic activity on the cultured newborn mouse cortical neurons.

关 键 词：去痴灵挥发油 血清药理学 皮层神经元 神经营养作用 

分 类 号：R285.5[医药卫生—中药学] R289.5[医药卫生—中医学]