乌灵菌粉对脑卒中后抑郁大鼠海马区单胺类神经递质及行为学改变的影响  

Effect of wulin powder on the changes of hippocampal monoamine neurotransmitter and behaviors in post-stroke depression rats

作　　者：裘涛[1] 陈眉[1] 陶水良[2] 代建峰[1] 吴乐程[1] 张宾辉[1] 

机构地区：[1]浙江省中医院神经内科,杭州310006 [2]浙江中医药大学基础医学系,杭州310053

出　　处：《中国新药杂志》2007年第11期857-860,共4页Chinese Journal of New Drugs

基　　金：浙江省中医药管理局课题(2004C029)

摘　　要：目的:观察乌灵菌粉对脑卒中后抑郁(PSD)大鼠的行为学改变及脑内单胺类神经递质的影响。方法:采用双侧颈总动脉永久性结扎后并给予行为限制的方法制作PSD大鼠模型,采用敞箱试验(open-field)观察大鼠行为学变化,并用高效液相法检测大鼠海马组织中4种单胺类神经递质去甲肾上腺素(L-NE)、多巴胺(DA)、5-羟色胺(5-HT)和5-羟基吲哚乙酸(5-HIAA)的含量,对比观察了乌灵菌粉组(0.3 g.kg-1,qd,ig,28 d)、盐酸氟西汀组(0.75 mg.kg-1,qd,ig,28 d)、模型组及假手术组的变化。结果:与假手术组比较,模型组行为学能力下降,脑内NE,DA,5-HT和5-HIAA下降(P<0.05);与模型组比较,乌灵菌粉组及盐酸氟西汀组行为学能力改善,脑内5-HT和5-HIAA水平上升(P<0.05),而两组间比较统计学差异不明显(P>0.05)。结论:乌灵菌粉可改善脑卒中后抑郁大鼠的行为学改变,其作用可能通过调节脑内5-HT水平。Objective： To observe the effect of wulin powder on the changes of hippocampal monoamine neurotransmitter and behaviors in post-stroke depression （PSD）rats. Methods： A PSD rat model was induced by permanent bilateral common carotid arteries ligation and a 7-day＇s limitation. The behavior was observed in an open field. Neurotransmitter contents in the hippocampi was detected by reversed-phase HPLC-fluorescence analysis. Results： Both the behavior ability and the level of NE,DA,5- HT,5-HIAA in PSD rats were decreased （P 〈 0.05, compared with sham-operated group）. Wulin powder as well as fluoxertine significantly improved the behavior ability and increased the level of 5-HT and 5-HIAA（P 〈 0.05）. There were no obviously difference between the two groups（P 〉 0.05 ）. Conclusion： The powder of wulin can improve PSD in rats via regulating 5-HT level in the brain.

关 键 词：乌灵菌粉 脑卒中后抑郁症 单胺类神经递质 行为学 

分 类 号：R285.5[医药卫生—中药学] R286.1[医药卫生—中医学]