白芍总苷对柯萨奇B_3病毒感染大鼠原代心肌细胞的血清药理学干预  

TGP interfere in primary rat myocardial cells with infection of Coxsackie virus B_3 by method of serum pharmacology

作　　者：李全礼[1] 都鹏飞[1] 王明丽[2] 

机构地区：[1]安徽医科大学第一附属医院儿科,合肥230022 [2]安徽医科大学微生物教研室,合肥230022

出　　处：《安徽医科大学学报》2007年第3期283-285,共3页Acta Universitatis Medicinalis Anhui

基　　金：安徽省教育厅资助项目(编号:2003kj172)

摘　　要：目的探讨白芍总苷(TGP)对柯萨奇B3病毒(CVB3)感染大鼠原代心肌细胞有无保护作用。方法取SD乳鼠心室肌制备大鼠原代心肌细胞,培养72h后,用不同剂量的TGP含药血清干预100TCID50CVB3感染心肌细胞,对比分析干扰素组、双黄连组、正常对照组。观察细胞病变,24h后测定细胞上清液中的cTnI、CK、LDH以及用MTT法检测细胞存活率。结果TGP高剂量组可减轻心肌细胞病变,减少cTnI、CK、LDH的释放。结论TGP对CVB3感染大鼠原代心肌细胞有保护作用。Objective To investigate the effects of total glucosides of paeony（TGP）for primary rat myocardial cells with infection of Coxsackie virus B3 （ CVB3 ）. Methods The primary rat myocardial cells from the ventricle muscle of newborn Sprague-Dawley rats were prepared. After 72 hours, the different TGP dose serum was added in myocardial cells with infection of CVB3 as well as Interferon group, Shuanghuanglian group, normal control group. These groups were respectively analyzed and compared by observing the cytopathogenic effect and mettled cTnⅠ, CK, LDH and survival rate after the myocardial cells were infected for 24 hours. Results The TGP group with high dose relieved the cytopathogenic effect, decreased cTnⅠ, CK, LDH of the cell supernatant. Conclusion TGP has protective action on the primary rat myocardial cells with infection of CVB3.

关 键 词：白芍/治疗应用 皂苷类/药理学 柯萨奇病毒感染/中药疗法 心肌/细胞学 

分 类 号：R282.71[医药卫生—中药学] R289.5[医药卫生—中医学]