黄芪提取物对Aβ_(25-35)所致阿尔茨海默病模型大鼠的学习记忆能力及海马神经元Bcl-2和Bcl-xl表达的影响  

Effect of the extract of astragalus on the ability of learning and memory and the expression of Bcl-2 and Bcl-xl protein of the hippocampal neurons in rat model of Alzheimer's Disease

作　　者：张云玲[1] 刘东梅[1] 吴庆四[2] 姚余有[2] 李卫平[1] 

机构地区：[1]安徽医科大学药理学教研室,合肥230032 [2]安徽医科大学公共卫生学院检验医学系,合肥230032

出　　处：《安徽医科大学学报》2007年第3期299-302,共4页Acta Universitatis Medicinalis Anhui

基　　金：安徽省自然科学基金课题(编号:00144414);安徽省高校省学术带头人科学研究资助(编号:2005hbz18)

摘　　要：目的观察黄芪提取物(EA)对Aβ25-35所致阿尔茨海默病(Alzheimer′sdiseases,AD)模型大鼠的学习记忆能力及脑内海马神经元Bcl-2和Bcl-xl表达的影响。方法采用双侧海马注射Aβ25-35制备AD大鼠模型,采用Morris水迷宫检测EA对模型大鼠认知功能的的影响;通过病理形态学观察EA对模型大鼠的脑保护作用;采用免疫组化法观察EA对模型大鼠海马神经元Bcl-2和Bcl-xl的表达的影响。结果EA(20、40、80mg/kg,ig×5d)能改善模型大鼠的学习记忆能力,减轻大鼠海马神经元的损伤,增强受损海马神经元Bcl-2和Bcl-xl的表达。结论EA能通过增强Bcl-2及Bcl-xl的表达,抑制海马神经元的凋亡,改善AD大鼠学习记忆能力。Objective To study the effects of the extract of astragalus on the expression apoptosis related gene Bcl- 2 and Bcl-xl of the hippocampal neurons in rat model of Alzheimer＇s disease induced by β-amyloid （Aβ） and it＇s learning and memory ability. Methods The AD model of rats was established by injecting Aβ25-35 into the CA1 district of both hippocampi. The effect of EA on learning and memory retention disorder in the model rats was studied by the test of Morris water maze ; the brain protection of EA in model rats was observed through pathomorphology changes; the expression of Bcl-2 and Bcl-xl of hippocampal neurons was detected by immunohistochemistry method. Results EA （20,40 and 80 mg/kg） could improved the ability of study and memory of AD rats and attenuate the hippocampi neuron injury induced by Aβ25-35 in rats. EA （20,40 and 80 mg/kg） could enhanced the expression of Bcl-2 and Bcl-xl. Conclusion EA （20,40 and 80 mg/kg） enhanced the expression of Bcl-2 and Bcl-xl to restrain the apoptosis of the hippocampal neurons, improve the learning and memory of AD rats.

关 键 词：黄芪 植物提取物/药理学 阿尔茨海默病/药物疗法 细胞凋亡 学习/药物作用 记忆/药物作用 原癌基因蛋白质C-BCL-2 

分 类 号：R282.71[医药卫生—中药学] R322.8[医药卫生—中医学]