单纯疱疹病毒2 gD蛋白T细胞表位区的预测及克隆  

Prediction and Clone of T Cell Epitope Region of HSV-2 gD Protein

作　　者：樊建勇[1] 杨慧兰[1] 关蕾[1] 王颖[1] 仕瑶慧[1] 

机构地区：[1]广州军区总医院皮肤科,广东广州510010

出　　处：《中国皮肤性病学杂志》2007年第6期330-333,共4页The Chinese Journal of Dermatovenereology

基　　金：国家自然科学基金资助项目(编号:30371290);广东省医学科研基金资助课题(编号:A2006492)

摘　　要：目的预测并克隆单纯疱疹病毒2 gD蛋白T细胞抗原表位集中区。方法根据相关计算机软件和互联网服务器进行表位预测;提取单纯疱疹病毒2基因组,用特异性引物扩增预测的gD蛋白T细胞抗原表位集中区基因,酶切后将目的基因插入载体pGEX-4T-1,对重组载体酶切及测序鉴定。结果预测得到了单纯疱疹病毒2gD蛋白T细胞抗原表位集中区,构建成功重组载体pGEX-gD,经测序确认序列正确。结论单纯疱疹病毒2 gD蛋白T细胞表位集中区的成功预测及克隆可为今后单纯疱疹病毒疫苗的研究打下基础。Objective To predict and clone the T cell epitope mass region of HSV-2 gD gene. Methods Epitope prediction was based on related software and internet servers, The genome DNA was isolated from HSV-2 virus and the the epitope gene was amplified by PCR. After enzyme digested, the epitope gene was inserted into pGEX-4T-1 plasmid. The recombinant plasmid pGEX-gD was confirmed by enzyme digested and DNA sequence analysis, Results The T cell epitope mass region of HSV-2 gD gene was obtained. The recombinant plasmid pGEX-gD was constructed successfully and the sequence of gD gene was correct. Conclusion The successful prediction and clone of T cell epitope mass region of HSV-2 gD protein gene provide a basis for study of HSV-2 vaccine.

关 键 词：单纯疱疹病毒 gD蛋白 表位 预测 

分 类 号：R752.1[医药卫生—皮肤病学与性病学] Q782[医药卫生—临床医学]