LRP16、E-cadherin、ER与PR在子宫内膜异位症中的表达及其意义  

Expression of LRP16,E-cadherin,ER and PR and their significance in endometriosis

作　　者：张燕[1] 孟元光[1] 陈美霞[1] 赵亚力[2] 韩为东[2] 田丽媛[2] 

机构地区：[1]解放军总医院妇产科,北京100853 [2]解放军总医院妇产科基础所分子生物室,北京100853

出　　处：《解放军医学杂志》2007年第6期632-635,共4页Medical Journal of Chinese People's Liberation Army

基　　金：国家自然科学基金资助项目(30471813);北京市自然科学基金项目(7052061)

摘　　要：目的探讨LRP16与雌激素受体(ER)、孕激素受体(PR)、E-钙黏附蛋白(E-cadherin)在子宫内膜异位症(EM)患者在位和异位内膜中的表达及其意义。方法采用免疫组化SP二步染色法,对72例EM患者在位和异位内膜中的LRP16、E-cadherin、ER、PR进行免疫组化染色。结果腺上皮细胞胞质中LRP16在在位内膜中的表达高于异位内膜(P<0.05);胞核中LRP16在位和异位内膜中的表达无明显差异;腺上皮细胞膜中E-cadherin在在位内膜中的表达低于异位内膜(P<0.05)。但在位和异位内膜中LRP16和E-cadherin的表达无相关性(P>0.05)。腺上皮细胞核中ER、PR在在位内膜中的阳性表达率高于异位内膜(P<0.05),异位内膜中PR的阳性表达率高于胞核中LRP16的阳性表达率(P<0.05),ER的阳性表达率低于LRP16,且ER与胞核中LRP16表达存在相关性。在位内膜胞核中LRP16的表达高于ER、PR(P<0.05)。结论LRP16和E-cadherin、ER、PR在在位和异位内膜中的表达异常可能与EM的发生发展有关。Objective To study the expression of LRP16 and E-cadherin （E-cad）, ER, PR in eutopic endometrium and ectopic endometrium of endometriosis （EM）, as well as to study the relation one another and the possible clinical significance. Methods The expression of LRP16 and E-cadherin, ER, PR in endometriurn was detected by immunohistochemical two step staining methods in 72 patients with endometriosis which was finally confirmed by laparoscopic operation. Results The expression of LRP16 in epithelioglandular endochylema in eutopic endometriurn of EM was significantly higher than that in ectopic endometrium （ P〈0. 05）, while no significant difference was found between expression level of LRP16 in epithelioglandular nucleus of eutopic endometrium and ectopic endometrium. The expression of E-cad in ectopic endometrium of EM patients was higher than in eutopic endometrium （ P〈0. 05）. No significant correlation existed between LRP16 and E-cad in both eutopic and ectopic endometrium. The expression of ER and PR in epithelioglandular nucleus in eutopic endometrium of EM patients was higher than in that of ectopic endometrium （ P〈0. 05）. The expression of PR was higher than the expression of LRP16 in ectopic endometriurn of EM patients （P〈0. 05）, the expression of ER was lower than the expression of LRP16 in ectopic endometrium of EM patients （P〈0. 05）, and the expression of ER was correlated with LRP16 in epithelioglandular nucleus of ectopic endometrium. The expression of both ER and PR was lower than that of LRP16 in eutopic endometrium of EM patients （ P〈0. 05）. Conclusion The expression levels of LRPI6, ER, PR and E-cad in epithelioglandular endochytema and nucleus were different in ectopic and eutopic endometrium of EM patients, and this abnormal expression may be correlated with pathogenesis and development of endometriosis.

关 键 词：E-CADHERIN 受体 雌激素 受体 孕激素 子宫内膜异位症 

分 类 号：R711.71[医药卫生—妇产科学]