碱性磷酸酶同工酶在老年女性骨质疏松诊断中的应用  

Application of alkaline phosphatase isoenzyme to the diagnosis of senile women with osteoporosis

作　　者：邓君[1] 饶绍琴[1] 洪华[1] 

机构地区：[1]四川省人民医院检验科,四川成都610071

出　　处：《实用医院临床杂志》2007年第4期42-43,共2页Practical Journal of Clinical Medicine

基　　金：四川省医学科学院四川省人民医院科研基金资助项目(980077);获四川省科技进步三等奖(2002-3-0182)

摘　　要：目的探讨碱性磷酸酶(ALP)同工酶在老年女性骨质疏松诊断中的应用。方法采用神经氨酸苷酶,用琼脂糖电泳法对体检正常者50例(对照组)和老年女性骨质疏松患者55例(骨质疏松组)血清中的ALP同工酶进行分离分析。结果经神经氨酸苷酶处理后,琼脂糖电泳法可以分离肝和骨ALP;两组肝型ALP比较,差异无显著性意义(P>0.05);骨型ALP比较,差异有显著性意义(P<0.05);热失活法和电泳法检测骨ALP具有显著的相关关系(r=0.92,P<0.05)。结论骨型ALP可为临床诊断老年女性骨质疏松提供可靠的依据。Objective To investigate the application of alkaline phosphatase isoenzyme to the diagnosis of senile women with osteoporosis. Methods Separated by neuraminidase, ALP isoenzyme in the serum of 50 normal women and 55 senile women with osteoporosis were analyzed by agarose electrophoresis. Results Liver and bone ALP could be separated by agarose electrophoresis after treatment by neuraminidase. There was no prominent difference in liver ALP between osteoporosis group and normal group（ P 〉 0. 05 ） , but there was difference in bone ALP（ P 〈 0.05 ）. A significant correlation was observed between heat denaturation and agarose electrophoresis to detect bone ALP（ r = 0. 92 ,P 〈 0. 05 ）. Conclusion Bone ALP can be used to diagnose senile women with osteoporosis.

关 键 词：肝型碱性磷酸酶同工酶 骨型碱性磷酸酶同工酶 神经氨酸苷酶 琼脂糖电泳法 老年女性 骨质疏松 

分 类 号：R589.5[医药卫生—内分泌] R446.11[医药卫生—内科学]