利用改进的一步法提取本地毛形线虫总RNA  

An improved single-step method for RNA isolation of Trichinella nativa

作　　者：郑宝亮[1] 付守申[2] 郜相君[2] 马辉[1] 郑鸣[1] 赵绪永[1] 郭宏伟[1] 杨向科[1] 

机构地区：[1]郑州牧业工程高等专科学校生物工程系,河南郑州450011 [2]大庆油田物业集团,黑龙江大庆163453

出　　处：《郑州牧业工程高等专科学校学报》2007年第2期1-2,5,共3页Journal of Zhengzhou College of Animal Husbandry Engineering

基　　金：河南省科技厅项目(0523010800)

摘　　要：利用胃蛋白酶消化小鼠肌肉,获取了旋毛虫种本地毛形线虫(T.nativa)的肌幼虫虫体。利用TRIZOL混合虫体直接提取肌幼虫的总RNA,通过分光光度计Ultrospec 3000检测结果,以及用RT-PCR方法扩增出了编码T.na- tiva 49 kDa的ES蛋白结构基因,后续试验结果显示改进的一步法提取本地毛形线虫总RNA效果很理想。The muscle--stage larvae of Trichinella nativa （T. nativa） were collected digested by pepsin- HC1. The total RNA of T. nativa was isolated using TRIZOL according to the manufacture＇s instruction after the worms being pestled together with TRIZOL. It was showed that from the results of RNA detection with Ultrospec 3000, and also from the next result that the gene of the worm which encoding 49kDa ES protein of T. nativa was amplified by RT--PCR, that the improved single--step method of RNA isolation for T. nativa we used was ideal.

关 键 词：TRICHINELLA nativa 肌幼虫 总RNA 

分 类 号：S855.9[农业科学—临床兽医学]