不同寄主植物的烟粉虱(Bemisia tabaci)内共生菌传毒相关GroEL蛋白的一致性  

Similarity of GroEL sequences from endosymbionts of Bemisia tabaci (Hemiptera: Aleyrodidae) in different host plants

作　　者：谭周进[1] 谢丙炎[2] 杨宇红[2] 郭建英 肖启明[1] 万方浩[3] 

机构地区：[1]湖南农业大学,湖南长沙410128 [2]中国农业科学院蔬菜花卉研究所,北京100081 [3]中国农业科学院植物保护研究所,北京100081

出　　处：《生态学报》2007年第6期2490-2497,共8页Acta Ecologica Sinica

基　　金：国家重点基础研究发展规划项目(2002CB111400)~~

摘　　要：通过对7种寄主植物上B型烟粉虱北京种群的内共生菌传毒相关groEL基因进行PCR扩增和测序,结合已有的相关序列,构建了groEL基因及其编码的GroEL蛋白的分子系统树。结果表明:烟粉虱内共生菌产生的groEL基因是一个非常保守的基因,北京不同寄主植物的烟粉虱内共生菌与IsraelB型烟粉虱内共生菌的groEL基因亲源关系非常近,位于同一进化分支,其编码的GroEL蛋白的分子系统树也基本上是一致的。不同物种的groEL基因及其编码的GroEL蛋白分别位于不同的分支,说明groEL基因及其编码的GroEL蛋白的分子系统树可以用于分析物种间的进化关系。氨基酸序列比较表明:烟粉虱内共生菌GroEL具有原核GroEL的保守氨基酸、ATP酶活性位点、多肽结合位点和GroES连接位点,为典型的hsp60。不同来源烟粉虱内共生菌GroEL有少数几个保守氨基酸发生了置换,可能不是GroEL功能的重要位点。说明在容易变异的细菌基因组中,groEL基因为了维持其正常重要的生理功能,会通过保持功能位点的稳定性来应对不同生态因素的影响。Endosymbionts included primary endosymbionts and secondary endosymbionts, accreting with insects. Endosymbionts evolved with insects and benefit from each other. Endosymbiont affected the insects＇ growth, insects＇ fertilizing and viral disease transmission of plants. GroEL, a 63 kDa protein encoded by the groEL gene of endosymbionts in Bemisia tabaci, played an important role in viral disease transmission by Bemisia tabaci. The product of the groEL gene was a type of viral binding protein. Viral particles that had reached the hemolymph interact with GroEL produced by endosymbionts on their way to the salivary glands Begomoviruses were transmitted solely by the and form a complex that protected whitefly Bemisia tabaci. Several whitefly virions from rapid proteolysis. species, such as Trialeurodes vaporariorum, were able to ingest Bemisia tabaci-transmissible begomoviruses but did not transmit them to plants. In order to probe into the invaded mechanism of Bemisia tabaci, cloning and sequencing of groEL of endosymbionts in Bemisia tabaci long-term living on different host plants in Beijing were conducted. Molecular phylogenetic trees, including groEL genes and GroEL proteins of endosymbionts in Bemisia tabaci and other bacteria, were reconstructed. The results showed that groEL was a very conservative gene and evolved very slowly. There was little difference among groEL genes from endosymbionts in different eco-types Bemisia tabaci. GroEL gene and GroEL protein from endosymbionts in different eco- types Bemisia tabaci belonged to the same branch. Molecular phylogenetic trees based on groEL and GroEL could explain phylogenetic relationships of different bacteria, but not for different eco-type species. There were identical molecular phylogenetic trees for groEL and GroEL. Comparison of GroEL sequences showed that： There were identical conservative amino acids, ATPase activity sites, polypeptide binding sites and GroES binding sites between GroEL of endosymbionts in Bemisia tabaci and other prokaryotic GroEL.

关 键 词：内共生菌 烟粉虱 GroEL蛋白 生态功能 蛋白质一级结构 

分 类 号：S433[农业科学—农业昆虫与害虫防治]