缺氧预适应小鼠海马脑区VEGF蛋白表达增加(英文)  

Hypoxic Preconditioning Increases Expression of VEGF in the Hippocampus of Mice

作　　者：邵国[1] 张彦波[2] 周卫华[3] 吕国蔚[4] 

机构地区：[1]包头医学院生物医学研究中心,内蒙古包头014010 [2]泰山医学院附属医院神经内科,山东泰安271000 [3]吉首大学医学院,湖南吉首416000 [4]首都医科大学低氧医学研究所,北京100054

出　　处：《吉首大学学报（自然科学版）》2007年第2期113-116,共4页Journal of Jishou University(Natural Sciences Edition)

基　　金：The National Natural Science Foundation of China(39670271);Beijing Natural Science Foundation(7962009)

摘　　要：目的观察重复低氧对小鼠海马组织内血管内皮生长因子(vascular endothelial growth factor,VEGF)蛋白质表达的影响.方法小鼠随机分为3组,分别暴露低氧0次(H0),1次(H1),4次(H4)后取海马组织,应用Western Blot技术,检测小鼠海马组织内VEGF的蛋白表达变化.结果实验发现,H0,H1和H4组海马组织内VEGF均表达,H1组与H0组之间没有差异,H4组显著升高(P<0.05,vs.H0 and H1).结论VEGF在急性低氧预适应小鼠海马组织表达增加可能参与预适应的形成及脑保护.Objective：To detect the effect of hypoxia preconditioning on vascular endothelial growth factor （VEGF） expression in acute repeated hypoxic mice hippocampus. Methods： Mice were divided into 3 groups randomly and were exposed to hypoxia for 4 runs （H4 group）, 1 run （H1 group） and 0 run （control group）. Western Blot was used to examine the VEGF level in mice hippocampus following exposure to hypoxia, total protein was extracted from mice hippocampus for Western blot. Results：VEFG were found in H0, H1 and H4 group. VEGF protein levels were found to be increased markedly in H4 （P 〈 0.05 ,vs. H1 and H0）. There is no difference between H1 and H0. Conclusion： The change of VEGF protein level is closely related to protection of the brain and the formation of preconditioning in hypoxic preconditioned mice.

关 键 词：缺氧预适应 海马 血管内皮生长因子 

分 类 号：R318[医药卫生—生物医学工程]