除草剂阿特拉津对血清体系中性激素干扰机理研究  

Disrupting Mechanism of Atrazine for Sex Hormones in Serum System

作　　者：李一菲[1] 郭栋生[2] 袁小英[2] 

机构地区：[1]太原科技大学,山西太原030024 [2]山西大学,山西太原030006

出　　处：《农业环境科学学报》2007年第3期1067-1070,共4页Journal of Agro-Environment Science

基　　金：太原科技大学校青年基金资助(2005009)

摘　　要：为了进一步了解环境内分泌干扰物对人体内性激素平衡的影响,选择阿特拉津这种已被确认为环境内分泌干扰物的农药,通过研究阿特拉津、天然性激素(雌二醇、睾酮)加入血清后体系紫外吸收峰的变化、温度与体系荧光强度关系等方法,确定了阿特拉津、天然性激素对血清组分的荧光猝灭机理,认为阿特拉津可发生类似天然性激素和血清组分间的基态络合。此外,通过计算各二元体系的结合常数发现,阿特拉津与血清组分的结合常数与天然性激素常数较为接近,可以认为阿特拉津具有与天然性激素相近的对血清组分的结合能力,并可能与天然性激素发生竞争结合,由此影响生物体内正常的性激素水平。In order to know further the disrupting effect of EDCs on the balance of sex hormones in human body, the interaction between atrazine and serum components was studied with ultraviolet spectroscopy and fluorescence analysis. The concentration-dependent variation of ultraviolet absorption peak and the temperature-dependent behavior of fluorescence intensity were investigated and compared with that of natural sex hormones （estradiol, testosterone） in serum system. The complexes were formed between atrazine （sex hormones） and serum components, and by using Lineweaver-Burk method, the binding constant K of estradiol, testosterone, and atrazine with serum components was calculated. The similar values of K suggested that the binding capability of atrazine with serum components was similar to that of sex hormones. Therefore, the normal binding between sex hormones and serum components can be disrupted by the competitively binding of atrazine, and the balance between estradiol and testosterone in human body might be disrupted.

关 键 词：环境内分泌干扰物 阿特拉津 性激素 荧光光谱法 

分 类 号：X503.1[环境科学与工程—环境工程]