阿魏酸钠对人心房肌细胞超快激活延迟整流钾电流的影响  

Effects of sodium ferulate on the ultrarapid delayed rectifier K^+ current in human atrial myocytes

作　　者：刘坤[1] 高翔[1] 刘金平[2] 张昌伟[2] 陈志坚[1] 曾秋棠[1] 廖玉华[1] 

机构地区：[1]华中科技大学协和医院心内科,武汉430022 [2]华中科技大学协和医院心外科

出　　处：《临床心血管病杂志》2007年第6期444-446,共3页Journal of Clinical Cardiology

摘　　要：目的:观察阿魏酸钠对人心房肌细胞超快激活延迟整流钾电流(IKur)的影响。方法:酶解法分离制备单个人心房肌细胞。采用全细胞膜片钳技术记录人心房肌细胞的IKur。比较加入阿魏酸钠前后IKur的变化。结果:0.4g/L阿魏酸钠不改变IKur电流-电压对应关系,0.4g/L阿魏酸钠对各个电压水平下IKur的电流密度(pA/pF)均有不同程度的抑制,+60mV时IKurpA/pF由4.99±0.35减小为3.33±0.26(P<0.05)。阿魏酸钠对IKur的抑制作用呈浓度依赖性,半数有效浓度IC50是(0.41±0.03)g/L;希尔系数是0.95±0.05。结论:阿魏酸钠作为一种钾通道阻滞剂,能有效地抑制人心房肌细胞IKur。Objective： To study the effects of sodium ferulate on the ultrarapid delayed rectifier K^＋ current （IKur） in human atrial myocytes. Method： Human atrial myocytes were isolated in an enzyme dispersion method. IKur in human atrial myocytes were recorded by using the whole cell patch clamp. Compare the changes of IKur in the absence and presence of sodium ferulate. Result：There was no effect of 0.4 g/L sodium ferulate on I-V relation of IKur and 0.4 g/L sodium ferulate inhibited IKur to some degrees at each test pulse. The current densities of IKor at ＋60 mV were reduced from 4.99±0.35 to 3.33±0.26（n=6, P〈0.05）. The inhibition was concentration-dependent. IC50 was 0.41±0.03 g/L and the Hill coefficient was 0.95±0.05. Conclusion：As a potassium channel blocker, Sodium ferulate can inhibit IKur in human atrial myocytes effectively.

关 键 词：阿魏酸钠 心房 钾通道 膜片钳 

分 类 号：R541.7[医药卫生—心血管疾病]