不对称二甲基精氨酸对体外培养内皮祖细胞数量和功能的影响  

Effects of asymmetrical dimethylarginine on number and activity of endothelial progenitor cells in vitro

作　　者：余华[1] 张怀勤[1] 肖方毅[1] 肖刚峰[2] 黄晓燕[1] 

机构地区：[1]温州医学院附属第一医院心内科温州医学院心血管生物和基因研究所,温州325000 [2]宁波市第二医院ICU科

出　　处：《临床心血管病杂志》2007年第6期454-456,共3页Journal of Clinical Cardiology

基　　金：浙江省自然科学基金资助项目(No:303648)

摘　　要：目的:观察不对称二甲基精氨酸(ADMA)对体外培养内皮祖细胞(EPCs)数量和功能的影响。方法:密度梯度离心法获取外周血单个核细胞,培养6d后,收集贴壁细胞并分别加入ADMA1、5、10μmol/L干预培养72h。激光共聚焦显微镜和流式细胞仪鉴定EPCs,分别观察EPCs的数量、增殖及黏附和产生一氧化氮的能力。结果:与对照组相比,不同浓度的ADMA可显著抑制外周血EPCs扩增、黏附增殖能力和产生NO的能力。结论:ADMA可抑制培养EPCs的数量和功能。Objective：To investigate whether asymmetrical dimethylarginine（ADMA） can reduce the number of endothelial progenitor cells（EPCs） and inhibit their function. Method, Mononuclearcells （MNCs） from peripheral blood were isolated by Ficoll density gradient centrifugation, and culturedin in vitro. After cultured six days, the attached cells were added with ADMA（1,5,and 10 μmol/L） for 72 hour. The cells were identified by confocal laser scanning microscope and flow cytometry. EPCs proliferation was assyed with CCK-8 kit assay. EPCs adhesion assay was performed by replating cells on fibronectin-coated dishes,and then adhereht cells were counted. NO production was assayed by Griess nitric oxide assay kit. Result：Compared with control group, ADMA can reduce the number of EPCs and inhibit the function of its proliferation, adhesion and excreting NO. Conclusion： ADMA can inhibit EPCs number and their function in vitro .

关 键 词：冠状动脉疾病 不对称二甲基精氨酸 内皮祖细胞 

分 类 号：R543.3[医药卫生—心血管疾病]