炭疽芽胞杆菌(Bacillus anthracis)检测质粒的构建及其应用  

Construction of Plasmid pBIB2006 for Detecting Bacillus anthracis and Its Application

作　　者：吕和平[1] 闵勇[2] 杨东明[1] 郑应华[1] 李旭[1] 武轶[1] 

机构地区：[1]北京生物医药研究所,北京100091 [2]华中农业大学农业微生物国家重点实验室,湖北武汉430070

出　　处：《微生物学杂志》2007年第3期38-41,共4页Journal of Microbiology

摘　　要：根据炭疽芽胞杆菌(Bacillus anthracis)毒性质粒pX01和pX02上的2个毒力相关基因cya和capA的序列特点,以pIJ2925为出发载体,采用一步重叠延伸PCR技术(One-step Overlap Extension PCR,简称OOE-PCR)构建了包含cya基因和capA基因保守区DNA片段的炭疽检测质粒pBIB2006。采用复合PCR对模拟炭疽危险品进行分析,结果表明pBIB2006可以为炭疽芽胞杆菌的检测提供准确、安全和方便的阳性参照品,从而为检测炭疽芽胞杆菌和炭疽芽胞杆菌灭活疫苗提供了便利。Plasmid pBIB2006 for detecting Bacillus anthracis （BA） containing 2 virulence-related genes cya and capA and their conservative area DNA fragments from BA poison plasmids respectively was constructed according to the sequence characteristics of the two genes with plJ2925 as starting carrier by one-step overlap extension PCR （ OOE-PCR）. Duplex PCR was adopted to carry out analyze the imitation of dangerous material of anthrax, the results indicated that pBIB2006 could provide accurate, safety, and convenient positive object of reference for BA detection, thereby, it provides convenience for BA detection and inactivated BA vaccine.

关 键 词：炭疽芽胞杆菌 cya基因 capA基因 OOE—PCR 

分 类 号：Q939.124[生物学—微生物学]