Effect of Electro-acupuncture on Relapse to Heroin-Seeking in the Heroin Self-administrating Rats  

作　　者：朱忠春 胡军 徐平 

机构地区：[1]Shanghai Research Institute of Acupuncture and Meridian, Shanghai 200030, P. R. China [2]Shanghai University of TCM, Shanghai 201203, R R. China

出　　处：《Journal of Acupuncture and Tuina Science》2007年第3期137-141,共5页针灸推拿医学（英文版）

基　　金：Supported by the National Nature Science Foundation （30271637） and the State Administration of TCM （02-03LQ05）

摘　　要：Objective： To observe the effect of electro-acupuncture on the relapse to heroin-seeking induced by heroin-related cues after withdrawal from heroin self-administration in rats. Methods： Sixty male Wistar rats were randomized into physiological saline group, withdrawal group A, withdrawal group B, electro-acupuncture （EA） treatment group A and EA treatment group B, with 12 rats in each group. The rats were trained by nose-poke response to self administer heroin （50μg/kg/per infusion） or saline with one daily 4-h session for 14 consecutive days, the session ended after 25 infusions were earned or 4 hours had passed, the reinforcement schedule was a progressive ratio. Then rats in withdrawal group A and withdrawal group B were withdrawn from heroin for 1 week and 2 weeks respectively, and then they were reintroduced to their training chambers for 4-h relapse testing which was induced by heroin-related environmental cues; And rats in EA treatment group A and EA treatment group B were also withdrawn from heroin for 1 week and 2 weeks respectively, during which they were given EA treatment for 20 min daily, then they received relapse testing; In the meantime, rats in saline group were trained with saline instead of heroin for 14 days as control, after 7 days of withdrawal from saline, they received relapse testing. Results： Following 7-8 days of drug training, the rats began to establish the stable intravenous heroin self-administration behavior; For the stable heroin self-administrating rats that received EA treatment, when they were reintroduced to their training chambers, their heroin-seeking behaviors were obviously inhibited, and this inhibitory effect related to the cumulativeness of EA stimulation. Conclusion： The results suggested that EA could prevent relapse to heroin-seeking to a certain extent.目的：观察电针对海洛因静脉自身给药大鼠复吸行为的影响。方法：60只Wistar雄性大鼠，随机分为生理盐水组、自然戒断A组、自然戒断B组、针刺治疗A组、针刺治疗B组，每组12只。采用强化程序为累进比率的方法训练大鼠通过鼻触反应进行海洛因静脉自身给药，每天训练4h，每次海洛因注射剂量为50μg／kg，限量每天25次，连续训练14天。然后自然戒断A组、B组分别停药7d与14d后进行复吸测试；针刺治疗A组、B组分别停药7d与14d，停药期间每天给予电针治疗，治疗结束后进行复吸测试；生理盐水组大鼠采用生理盐水代替海洛因训练，连续14d，停药7d后进行复吸测试。结果：大鼠经7-8天的自身给药训练，都开始建立起稳定的静脉自身给药行为，对于已形成稳定自身给药行为的大鼠，在停药戒断期间给予电针治疗，当大鼠再次返回实验笼时，其觅药行为明显被抑制，且抑制程度与电针治疗的累积量相关。结论：电针具有一定的防复吸潜力。

关 键 词：Drug Self-Administration Drug Dependence HEROIN Relapse to Drug-seeking Acupuncture Therapy ELECTRO-ACUPUNCTURE 

分 类 号：R246.2[医药卫生—针灸推拿学]