Endostatin-Angiostatin融合基因治疗大鼠C6胶质瘤的3.0T磁共振动态观察  

Investigation of tumor response after Endostatin-Angiostatin fusion gene therapy in a C6 rat glioma model by a 3.0T MR scanner

作　　者：沈慧聪[1] 戴建平[1] 高培毅[1] 马军[1] 李少武[1] 艾林[1] 魏新华[1] 

机构地区：[1]首都医科大学附属北京天坛医院神经影像中心,北京100050

出　　处：《中国医学影像技术》2007年第6期805-808,共4页Chinese Journal of Medical Imaging Technology

摘　　要：目的探讨3.0T磁共振在重组单纯疱疹病毒介导的Endostatin-Angiostatin(Endo-Angio)融合基因对大鼠C6胶质瘤疗效评价中的作用。方法30只雄性Wistar大鼠采用立体定向方法在颅内接种C6细胞,将荷瘤大鼠随机分为两组,治疗组与对照组;两组大鼠分别于接种后1、2、3周进行MR检查;第2、3周检查结束后每组留取2只鼠脑标本进行病理学检查;治疗组大鼠于第1周检查后于肿瘤接种位置原位注入Endo-Angio融合基因进行治疗。结果第1周检查共25只大鼠成瘤(成功率83%),第2周两组肿瘤体积均增大,二者无统计学差异(P>0.01),病理检查显示治疗组大鼠微血管腔减少,间质水肿及细胞水肿较对照组明显严重;第3周时治疗组大鼠体积减小,小于对照组(P<0.01)。结论重组单纯疱疹病毒介导的Endo-Angio融合基因对大鼠C6胶质瘤的生长有抑制作用,3.0T磁共振可以较好地动态观察大鼠C6胶质瘤的生长及治疗变化。Objective To evaluate the use of 3.0T MR imaging for detection of tumor response to Endostatin-Angiostatin （Endo-Angio） fusion gene therapy in a C6 rat tumor model. Methods Thirty male Wistar rats with C6 tumor cells implan ted intracranially into the striatum were divided into two groups： treatment and controls. Treated rats received recombinant herps simplex viral （R-HSV） mediated Endo-Angio fusion gene therapy on day 8, when the tumor were conformed on MRI. MR examinations were acquired on 1, 2, 3 week after implantation. Two rats were sacrificed to perform the histopathological examination after examinations of week 2 and 3. Pretreatment and posttreatment tumor volumes were measured on post- contrast MR imaging. Results On MRI, 25 rats （12 in control and 13 in treatment group） demonstrated tumor on week 1. The tumors in both control and treatment group continued to increase in size from week 1 to week 2, there was no significant difference in volumes of the two groups （P〉0.01）. H ＆E stained slices showed more prominent tumor interstitial edema and swelling of tumor cell in the treated rats than the controls. The tumors of the treated rats showed slowly growth in size on week 3, which was different to the controls （P〈0.01）. Conclusion Endo-Angio fusion gene had inhibited the growth of the rat C6 glioma. 3.0T MRI is useful for monitoring tumor progression and following therapy.

关 键 词：大鼠 胶质瘤 基因疗法 ENDOSTATIN Angiostatin磁共振成像 

分 类 号：R445.2[医药卫生—影像医学与核医学] R739.41[医药卫生—诊断学]