集合HIV RNA RT-PCR方法验证及其在IDUs中窗口期检测的应用  

Validation and application of pooled HIV RNA RT-PCR in detecting HIV window period of IDUs

作　　者：潘品良[1] 许文燕[1] 张麒[1] 陶晓霞[1] 毛玮[1] 佐合拉[2] 孙显光[3] 秦光明[4] 蒋岩[1] 

机构地区：[1]中国疾病预防控制中心性病艾滋病预防控制中心,北京100050 [2]新疆维吾尔自治区疾病预防控制中心,乌鲁木齐830011 [3]贵州省疾病预防控制中心,贵阳550000 [4]四川省疾病预防控制中心,成都610031

出　　处：《中国艾滋病性病》2007年第3期198-200,共3页Chinese Journal of Aids & STD

基　　金：国家"十五"科技攻关课题基金资助项目(2001BA705B01)

摘　　要：目的对已建立的集合艾滋病病毒(HIV)RNA逆转录-聚合酶链反应(RT-PCR)方法进行实验室验证,并将其应用于静脉吸毒人群中窗口期的检测。方法采用50∶1、10∶1、1∶1三级集合HIV RNA RT-PCR方法进行盲样验证及可重复性验证。结果(1)集合HIV RNA RT-PCR方法盲法验证的结果和预期值一致,重复性验证中9个一级集合的病毒载量对数值的变异系数为6%,单因素方差分析P>0.05,方法的重复性良好。(2)检测3 570份吸毒人群的HIV抗体阴性血清或血浆样本。对发现的RNA阳性感染者进行追踪采样,发现2例HIV RNA窗口期阳性。追踪随访,HIV-1抗体阳转。结论集合HIV RNA RT-PCR方法具有良好的实验性能,可应用于HIV高危人群窗口期检测。Objective To validate the method of pooled HIV RNA RT-PCR and to apply it in detecting HIV window period of IDUs. Methods Pooled HIV RNA RT-PCR（3-staged pooling by 50：1,10：1,1：1 and Roche RT-PCR）was validated in blind samples. Results Result of pooled HIV RNA RT-PCR blind validation was consistent with the expected value. Reproducibility of pooled HIV RNA RT-PCR was good with CV（coeffident of variation）as 6% and P〉0.05. The serum or plasma samples from 3 570 intravenous drug users（ IDUs）were tested, which were negative HIV third-generation antibody assays. Two samples were found to be HIV RNA positive. Acute infection was followed-up to sero-conversion. Its HIV antibody was positive in subsequent samples of XJ. Conclusion After the validation, pooled HIV RNA RT-PCR is a good method of testing reprodudbility, and can be applied to screen HIV window-period infection in high-risk groups of population.

关 键 词：艾滋病病毒 窗口期 逆转录-聚合酶链反应 

分 类 号：R373.9[医药卫生—病原生物学]