双气消滞散对G豚鼠致石后胆囊调宁蛋白的影响  

Effect of Shuangqi-xiaozhi powder on Calponin in gallbladder of guinea pigs with gallstones

作　　者：郑嘉岗[1] 方邦江[1] 

机构地区：[1]上海中医药大学附属龙华医院消化科,上海200032

出　　处：《中国中西医结合消化杂志》2007年第3期148-150,共3页Chinese Journal of Integrated Traditional and Western Medicine on Digestion

基　　金：国家自然科学基金资助项目(30672698);上海市重点学科建设项目(70304)

摘　　要：[目的]探讨双气消滞散促进胆管动力的作用机制。[方法]将受试豚鼠随机分为4组,即正常组、模型组、双气消滞散(中药)组、熊去氧胆酸(西药)组。后3组采用高胆固醇致石食饵诱发法建立豚鼠胆结石动物模型,并使其发生继发性胆囊运动功能障碍,其中中药组、西药组分别给予相应药物进行实验性治疗,连续7周后,分别对引起实验性豚鼠胆囊运动功能障碍的胆囊组织调宁蛋白(Cap)表达进行观察。[结果]中药组能有效促进胆囊动力(与模型组比较P<0.01),并且其效果优于西药组(P<0.01)。模型组动物胆囊组织Cap表达水平较正常组显著升高(P<0.01),中药组和西药组胆囊组织Cap表达水平虽均高于正常组(分别P<0.01、<0.05),但与模型组比较,其水平均明显降低(均P<0.01),且中药、西药2组胆囊组织Cap表达水平比较差异无统计学意义(P>0.05)。[结论]双气消滞散能显著促进胆囊动力,其作用机制可能与下调胆囊平滑肌组织Cap水平,提高胆囊平滑肌收缩能力有关。[Objective]To explore the mechanisms of Shuangqi-xiaozhi powder on dynamic of biliary tract. [Methods]The guinea pigs were randomly divided into 4 groups, which were normal control group, untreated group, Shuangqi-xiaozhi powder-treated group and ursodeoxycholic acid （UDCA）-treated group. The guinea pigs of the latter 3 groups were induced gallstones by high-cholesterol diet. UDCA and Shuangqi-xiaozhi powder were given to the guinea pigs in the corresponding group respeciively for seven weeks. The gallstone formation and the expression of Calponin in gallbladder were observed. [Results] The Shuangqi-xiaozhi powder was able to promote the dynamic of gallbladder significantly （P〈0. 01, vs untreated group）, which was more effective than UDCA （P〈0. 01）. The expression of Calponin in untreated group was significantly in- creased as compared with that in normal control （P〈0. 01）. Although the expression of Calponin in Shuangqi-xiaozhi powder treated group or UDCA-treated group increased as compared with that in normal control group （P〈0. 01, P〈0. 05）, they decreased as compared with that in un- treated group （P〈0. 01）. There was no significant difference on the expression of Calponin between Shuangqi-xiaozhi powder-treat group and UDCA-treated group （P〈0. 05）. [Conclusion ] Shuangqi-xiaozhi powder may promote the dynamic of gallbladder significantly. Its mechanism may be related to down-regulating the expression of Calponin in the smooth muscle of gallbladder and enhance the contractibility of smooth muscle in gallbladder.

关 键 词：胆结石 双气消滞散 胆囊动力学 调宁蛋白 

分 类 号：R285.5[医药卫生—中药学]