β-榄香烯对肝星状细胞凋亡相关蛋白Bcl-2家族的影响  

Effects of β-elemene on the expression of Bcl-2 family in hepatic stellate cells

作　　者：朱清静 熊振芳[2] 杨玲[3] 

机构地区：[1]武汉市传染病医院中西医结合肝病重点专科,湖北武汉430022 [2]湖北省中医院肝病科,湖北武汉430061 [3]华中科技大学同济医学院附属协和医院消化内科,湖北武汉430022

出　　处：《中国中西医结合消化杂志》2007年第3期151-152,155,共3页Chinese Journal of Integrated Traditional and Western Medicine on Digestion

基　　金：国家自然科学基金资助项目(30500658);湖北省自然科学基金资助项目(2004ABA236);湖北省教育厅科研基金课题(D200516014)

摘　　要：[目的]研究β-榄香烯对肝星状细胞(HSC)凋亡及凋亡相关蛋白的影响。[方法]传代培养的大鼠HSC系HSC-T6与不同剂量β-榄香烯(终浓度为10、5、2.5 mg/L)共同培养48 h后,应用Annexin-V-碘化丙锭染色检测β-榄香烯对HSC凋亡的影响,免疫组化检测凋亡相关蛋白BcL-2/Bax。[结果]β-榄香烯可使HSC凋亡率明显增多,同时可使凋亡抑制分子Bcl-2表达下调,促凋亡分子Bax表达增强(P<0.01)。[结论]β-榄香烯可通过下调Bcl-2/Bax比率,促进HSC凋亡。[Objective]To investigate the effect of β-elemene on apoptosis and Bcl-2/Bax expression of the hepatic stellate cells（HSC） in vitro. [Methods]The rat hepatic stellate cells line- HSC-T6 was incubated with various concentrations of beta-elemene for 48 hours. The apoptosis was examined with Annexin V-Propidium iodide assay, and the expression of Bax, Bcl-2 was detected with cell immunocytochemistry stain. [-Results] β-elemene could markedly induce the apoptosis of the cells with Bcl-2 protein expression down-regulated and Bax protein expression up-regulated（P〈0. 01）. [Conclusion ]β-elemene could down regulate the ratio of Bcl/Bax to increase the sensitivity of apoptosis and induced HSC apoptosis.

关 键 词：Β-榄香烯 肝纤维化 肝星状细胞 凋亡 

分 类 号：R322.4[医药卫生—人体解剖和组织胚胎学]