环孢菌素A两种不同产生菌原生质体的制备和再生  

Formation and regeneration of protoplasts from two cyclosporin A producing fungus species

作　　者：林永勉[1] 郑孝贤[1] 郑卫[1] 程元荣[1] 

机构地区：[1]福建省微生物研究所,福州350007

出　　处：《中国抗生素杂志》2007年第6期337-340,共4页Chinese Journal of Antibiotics

基　　金：福建省自然科学基金资助项目C0010020

摘　　要：以环孢菌素A两种不同产生菌——雪白白僵菌和茄病镰刀菌为出发菌株,开展了原生质体制备和再生条件的研究,并分别实现了两个不同种的菌株原生质体再生。Protocols for formation and regeneration protoplasts for two different fungus species, Beauveria nivea and Fusariurn solani, were described. High yield of protoplasts was obtained by using a combined enzyme system containing lywallzyme and snailase. The effects of factors such as osmotic stabilizers, cultiva- tion time, temperature, pH, incubation time on the formation and regeneration were studied.

关 键 词：环孢菌素A 雪白白僵菌 茄病镰刀菌 原生质体 

分 类 号：R979.5[医药卫生—药品]