荚膜在新生隐球菌致人脐静脉内皮细胞损伤中的作用  

Role of Cryptococcus neoformans capsule in Cryptococcus neoformans-induced damage of human umbilical vein endothelial cells

作　　者：曹艳云[1] 徐顺明[1] 汪晓军[2] 温海[2] 

机构地区：[1]上海市浦东新区人民医院皮肤科,上海201200 [2]第二军医大学长征医院皮肤科,上海200003

出　　处：《第二军医大学学报》2007年第2期148-150,共3页Academic Journal of Second Military Medical University

基　　金：国家自然科学基金(30471566)~~

摘　　要：目的探讨无荚膜和有荚膜(野生株)两种新生隐球菌菌株对人脐静脉内皮细胞(HUVEC)抑制率的差异,明确荚膜在其中发挥的作用。方法通过透射电子显微镜(TEM)观察新生隐球菌侵入和损伤HUVEC的过程以及作用后细胞的形态学变化;分别在不同时相点用细胞计数试剂盒-8(CCK-8)检测细胞抑制率的变化。结果通过TEM观察到两种菌株均造成细胞不同程度的损伤细胞的内质网疏松,线粒体肿大,细胞核膜损伤及其细胞的基本结构紊乱。CCK-8试剂盒的检测结果表明,共孵30min时,两菌株对HUVEC的抑制率比较无统计学意义;共孵1、2、3、4h时,无荚膜株对内皮细胞的抑制率均高于野生株(P<0.05),在1h无荚膜株的抑制率可高达79%;5h时,两菌株对HUVEC的抑制率无统计学差异。结论无荚膜株较野生株造成HUVEC更大程度的损伤,说明荚膜在隐球菌损伤HUVEC过程中发挥着重要作用。Objective：To study the different inhibitory effects of capsule-depleted and wild type Cryptococcus neoformans （C. neoformans） on human umbilical vein endothelial cell （HUVEC）, so as to clarify the role of C. neoformans capsule. Methods： The infection process of HUVEC by C. neoformans and the cellular morphologic changes of HUVEC were observed by transmission eletron microscope （TEM）. The cell inhibition rates were detected with CCK-8 kit at different phases. Results： TEM observation revealed different degrees of cell damages after co-cultured with the above 2 strains of C. neoformans, with the loose endoplasmic reticulum, swollen mitochondria, nuclei damage, and disarranged cell structure. The result of CCK-8 showed that there was no significant difference in the inhibition rates of HUVEC between the 2 groups 30 min after co-culture （P〉0. 05）. 1 h, 2 h , 3 h, and 4 h after co-culture, the inhibition rate in capsule-depleted group was higher than that of the wild strain group（P〈0.05）, with the inhibition rate being 79% at 1 hour. Five hours after co-culture, the inhibition rates were similar in capsule-depleted group and wild strain group（P〉0.05）. Conclusion： Capsule-depleted C. neoformans causes more significant endothelial cell injury than wild strain C. neoformans does, indicating that the capsule play an important role in C. neoformans-induced damage of endothelial cells.

关 键 词：新生隐球菌 人脐静脉内皮细胞 英膜 

分 类 号：R379.5[医药卫生—病原生物学]