臭氧对胎牛血清氧化损伤的表面增强拉曼光谱  

Surface-Enhanced Raman Spectra of Oxidation Damnification of Fetal Bovine Serum by Ozone

作　　者：邹祖全[1] 刘燕楠[2] 吴瑛 李奇楠[2] 陈梅宗[1] 孙海英[1] 李任植[1] 

机构地区：[1]南开大学物理科学学院生物物理系,天津300071 [2]南开大学物理科学学院光子学中心 [3]国家纳米技术与工程研究院,天津300457

出　　处：《光谱学与光谱分析》2007年第6期1140-1142,共3页Spectroscopy and Spectral Analysis

基　　金：南开大学杰出学者基金项目(PO2003)资助

摘　　要：对实验室细胞培养用的国产胎牛血清在臭氧作用前后进行了表面增强拉曼光谱测试,范围200～1800cm-1。发现臭氧作用1h后拉曼谱线强度大大减弱,作用3h后谱线强度进一步减弱,但减弱的程度明显减慢,说明臭氧氧化作用具有快速的特点。臭氧作用下蛋白质主链的有序构象(α-螺旋,β折叠)明显减少,对β折叠的损伤更为严重,β回折几乎消失。芳香族氨基酸和半胱氨酸侧链在臭氧作用下氧化损伤非常明显。这些特征谱线的变化,反映了臭氧的氧化作用使蛋白质变性,构象变化以及降解。Fetal bovine serum was treated by ozone for 1 hour and 3 hours before getting its surface-enhanced Ram.an spectra from 200 to 1 800 cm^-1. Treated with ozone for 1 hour, it shows a significant decrease in band intensity. Treated with ozone for 3 hours, the band intensity has a further decrease but not so obviously, which means that oxidation of ozone is short lived. Treated with ozone, the orderly conformations of main chains in protein such as α-helix, β-sheet and β-comer are damaged seriously. Aromatic side chains and C-S of Cys and Met also are damnified greatly. All this means that strong oxidation of ozone results in denaturation, conformational changes and even degradation in protein.

关 键 词：表面增强拉曼光谱 胎牛血清 臭氧 

分 类 号：O657.3[理学—分析化学]