丹参单体对TNF-α诱导大鼠动脉平滑肌细胞NF-κB和ICAM-1的影响  

Influence of salvia miltiorrhiza monomer on expressions of NF-κB and ICAM-1 in rat VSMC induced by TNF-α

作　　者：范英昌[1] 姜希娟[1] 赵桂峰[2] 卢斌[3] 杨琳[3] 

机构地区：[1]天津中医药大学病理教研室,天津300193 [2]天津中医药大学第一附属医院 [3]天津中医药大学实验教学部

出　　处：《北京中医药大学学报》2007年第6期380-383,I0003,共5页Journal of Beijing University of Traditional Chinese Medicine

基　　金：天津市自然科学基金资助项目(No.043111211)

摘　　要：目的观察丹酚酸B和丹参酮ⅡA对肿瘤坏死因子(TNF-α)诱导的血管平滑肌细胞(VSMC)核因子κB(NF-κB)和细胞间黏附分子(ICAM-1)表达的影响,探讨丹参单体抗动脉粥样硬化(AS)的作用机制。方法体外培养大鼠主动脉VSMC;RT-PCR法和免疫细胞化学法检测VSMCICAM-1mRNA和ICAM-1蛋白的表达;细胞ELISA和免疫细胞化学法检测NF-κB的表达。结果TNF-α上调VSMCICAM-1mRNA和ICAM-1蛋白的表达,提高NF-κB水平(P<0.01)。两种丹参单体都抑制上述因子的表达,以丹酚酸B效果更佳。结论丹参单体抗AS的机制之一是抑制炎症相关因子的表达,丹酚酸B抗炎效果优于丹参酮ⅡA。Objective To observe the influence of salvianolie acid B and tanshinone Ⅱ A on the expressions of nuclear factor kappa B （ NF-κB ） and intercellular adhesion molecule-1 （ ICAM-1 ） in cultured vascular smooth muscle cells （VSMC） induced by tumor necrosis factor-α （TNF-α） , and to investigate the molecular mechanism of salvia miltiorrhiza monomer against atherosclerosis （AS）. Methods The rat VSMC were cultured in vitro, the mRNA and protein expression of ICAM-1 were observed by RT-PCR and immunocytochemistry technique respectively. The NF-κB expression was detected by both of immunocytochemistry technique and cell ELISA. Results The mRNA and protein expression of ICAM-1 were up-regulated and NF-κB expression was also significantly increased by TNF-α （ P 〈 0.01 ）. Two kinds of salvia mihiorrhiza monomer markedly inhibited the expressions of NF-κB and ICAM-1. Salvianolic acid B had a better effect than tanshinone Ⅱ A. Conclusion One of the molecular mechanisms of salvia mihiorrhiza monomer against AS is that it can inhibit the expressions of inflammation-related factors in VSMC. Salvianolic Acid B has a better effect in anti-inflammation than tanshinone Ⅱ A.

关 键 词：肿瘤坏死因子 细胞间黏附分子-1 核因子ΚB 丹酚酸B 丹参酮ⅡA 

分 类 号：R285.5[医药卫生—中药学]