心叶球兰组织培养与快速繁殖试验  

Hoya kerrii in Vitro and Its Rapid Propagation

作　　者：管艳[1] 肖三元[1] 梁国平[1] 

机构地区：[1]云南省热带作物科学研究所,云南景洪666100

出　　处：《热带农业科技》2007年第2期41-43,共3页Tropical Agricultural Science & Technology

摘　　要：以心叶球兰叶片为外植体,愈伤组织诱导和继代增殖培养基MS+6-BA2.0mg.L-1+NAA0.5mg.L-1+2,4-D1.0mg.L-1+GA1.0mg.L-1+白糖3%,诱导的愈伤组织多,生长旺盛,增殖倍数为2～3倍;丛芽诱导培养基MS+KT0.5mg.L-1+NAA0.5mg.L-1+GA1.0mg.L-1+白糖3%,芽的诱导率为79.3%;在丛芽增殖及复壮培养基1/2MS+IBA1.0mg.L-1+白糖2%上,有20%～30%玻璃化丛芽转为绿色苗,玻璃化单芽经复壮培养长成绿色苗;诱导生根培养基为1/2MS+IBA0.5mg.L-1+白糖2%,玻璃化生根率为56.4%,淡绿色苗生根率达91.1%。瓶苗移栽基质泥炭土∶珍珠岩∶甘蔗渣(1∶1∶1)的成活率最高,达91.4%。Take the leave of Hoya kerrii as explant, the optimum medium for more induce callus and inherit multlphcatlon was MS＋6-BA 2.0mg/L＋NAA0,5 mg/L＋2,4-D1.0 mg/L＋GA1.0 rag/L＋3% sugar, with strong growth and 2-3 times of proliferation; Induction of cluster bud reaches to 79.3% in the medium of MS＋KT0.5mg/L＋NAA0.5mg/L＋GA1.0mg/L＋3% sugar; Vitrification of shoots in the medium for proliferation and regenation of 1/2MS＋IBA1.0mg/L＋2% sugar turned to green one （20%-30%）; Induction of roots is in medium of 1/2MS＋IBA0.5mg/L＋2% sugar, rooting rate of vitrification and light green shoots were 56.4% and 91.1% respectively,The survival rate of bottle shoots in media of mud：pearlite：sugarcane waste （1：1：1） was the highest （91.4%）.

关 键 词：心叶球兰 组织培养 快速繁殖 玻璃化苗 

分 类 号：S682.2[农业科学—观赏园艺]