日本血吸虫双价DNA疫苗pVIVO2-Sj14-Sj23免疫方案的研究  

Studies on the optimal immunization of Schistosoma japonicum bivalent DNA vaccine

作　　者：艾熙[1] 夏丽霞[1] 谢姣[1] 胡媛[2] 石佑恩[2] 

机构地区：[1]华中科技大学同济医学院,02级七年制学生武汉430030 [2]华中科技大学同济医学院病原生物学系

出　　处：《中国血吸虫病防治杂志》2007年第3期175-178,共4页Chinese Journal of Schistosomiasis Control

基　　金：国家高技术研究发展计划(863计划)(2004AA2Z3212)

摘　　要：目的研究双价DNA疫苗pVIVO2-Sj14-Sj23在不同免疫剂量、免疫次数、免疫有效时间及不同免疫途径的最佳免疫方案。方法构建和制备双价DNA疫苗pVIVO2-Sj14-Sj23;110只雄性BALB/c小鼠随机分成11组,每组10只,按照四因素三水平分成2个对照组和9个实验组。各组统一在末次免疫后30d每鼠感染(40±2)条日本血吸虫尾蚴,感染后45d剖杀,计数成虫虫荷。结果双价DNA疫苗9个实验组获得40.14%～62.68%的减虫率。通过SPSS12.0进行正交设计的统计分析:F值<F′临界值,P>0.05。免疫剂量,免疫次数,免疫途径和免疫有效时间几个因素间差异无统计学意义。结论实验结果提示该疫苗的较好免疫方案为:50μg/只,免疫2次,采取皮内注射,免疫有效时间至少为12周。Objective To study the optimal immunization of bivalent DNA vaccine encoding Schistosorna japonicurn 14 kDa （Sjl4） and 23 kDa （Sj23） with the different immunized dosages, inoculated times, inoculated routes and challenge time after the last immunization. Methods Construction and preparation of the bivalent DNA vaccine of Schistosorna japonicurn 14 kDa（Sjl4） and 23 kDa（Sj23） were porformed. A total of 110 male BALB/c mice, about 5-6 weeks old, were randomly divided into 11 groups, 2 were the control groups and 9 were the experimental groups, designed with four factors in orthogonal trial. Each mouse of the groups was infected with （40± 2） cercariaes of Schistosorna japonicurn in the 30 th day after last immunization, and 45 days after the infection, the mice were sacrificed, and the burden of the adult worms was invetigated. Results There was no significant difference in the impact on immunity protection excited by the different factors and different levels of each factor （P〉0.05）. Conclusion According to this experiment, the optimal immunization for this vaccine is 50μg per mouse, twice immunization and intradermal injection, and the period of efficacy is at least 12 weeks after the challenge.

关 键 词：日本血吸虫 双价DNA疫苗 Sj14 SJ23 免疫方案 

分 类 号：R383.24[医药卫生—医学寄生虫学]