骨髓间充质干细胞成肌诱导分化的实验研究  

Experimental Study of Inducing Differentiation of Mesenchymal Stem Cells Differetiated into Myoblasts

作　　者：姚瑶[1] 何等旗[1] 唐休发[1] 华成舸[1] 冯杨[1] 李小玉[1] 王斯华[1] 

机构地区：[1]四川大学华西口腔医学院头颈肿瘤外科,成都610041

出　　处：《口腔颌面外科杂志》2007年第2期126-129,共4页Journal of Oral and Maxillofacial Surgery

基　　金：教育部博士学科点基金资助(批准号:20050610077)

摘　　要：目的:观察骨髓间充质干细胞(mesenchymal stem cells,MSCs)经5-氮杂胞苷(5-azacytidine,5-Aza)诱导的成肌向分化潜能。方法:分离纯化4周龄SD大鼠的MSCs,以5-Aza诱导,通过细胞形态、免疫组织化学染色观察诱导后不同时相点的细胞。结果:经5-Aza诱导后14d,细胞表达结蛋白(desmin)。细胞形态和排列方式发生明显变化,可见有肌管样细胞出现。结论:MSCs经5-Aza诱导后可以向骨骼肌成肌细胞定向分化。Objective： To examine the potential of bone marrow mesenchymal stem cells （MSCs） induced into skeletal muscle ceils exposed to 5-azacytidine. Methods： 4-week old rats MSCs were separated and purified, and then induced by 5-azacytidine. Morphological change and immunohistochemisty were observed at various intervals. Results： MSCs began to express desmin after induction by 5-azacytidine at 14th day. The morphology and alignment of the cells changed obviously. The myotubule-like cells were observed. Conclusion： Myoblasts （skeletal muscle） could be differentiated from MSCs under the induction of 5-azacytidine.

关 键 词：骨髓间充质干细胞 5-氮杂胞苷 成肌细胞 诱导分化 

分 类 号：Q813[生物学—生物工程]