丹参酮Ⅱ_A微乳的制备与质量评价  

Preparation and quality evaluation of tanshinone Ⅱ_A microemulsion

作　　者：王高森[1] 侯世祥[1] 毛声俊[1] 梁栗[1] 王觉晓[1] 黄利[1] 唐晓欢[1] 

机构地区：[1]四川大学华西药学院,四川成都610041

出　　处：《华西药学杂志》2007年第3期279-282,共4页West China Journal of Pharmaceutical Sciences

摘　　要：目的研制丹参酮ⅡA微乳,并评价其质量。方法通过滴定法绘制伪三元相图,筛选微乳处方;采用RP-HPLC法测定丹参酮ⅡA的含量,以外观、粒径及其分布、Zeta电位、含量为指标考察微乳的稳定性。结果优选的处方含聚氧乙烯蓖麻油-大豆磷脂-无水乙醇-油酸乙酯(2:1:2:5)和水,所制微乳的外观均一透明,平均粒径22.1 nm,多分散指数0.262,Zeta电位-2.73 mV,载药量748.4μg.ml-1。结论所制微乳的理化性质较稳定,为细胞药理学及体内研究奠定了基础。OBJECTIVE To prepare and evaluate tanshinone ⅡA microemulison（Tan - ⅡA - ME）. METHODS The formulation was investigated by pseudoteruary phase diagram obtained by titration method. Tan - ⅡA was determined by RP - HPLC method and physical appearance, particle size with distribution, Zeta potential and contents of Tan - ⅡA were used as indexes to evaluate the stability of Tan - ⅡA ME. RESULTS The optimized formulation was composed of Cremophor EL/soybean phospholipid mixture - dehydrated alcohol -ethyl oleate -water （2 ： 1 ： 2 ： 5 ）. For Tan - ⅡA ME, the physical appearance was transparent and uniform. The average diameter was 22.1 nm with a polydispersity index of 0.262, the Zeta potential was - 2.73 mV, and the drug loading amount was 748.4μg. ml - i. CONCLUSION The physicochemical properties of Tan - ⅡA ME were relatively stable, which laid a foundation for further study of cytopharmacology and in vivo disposition.

关 键 词：丹参酮ⅡA 微乳 伪三元相图 高效液相色谱法 

分 类 号：R94[医药卫生—药剂学]