HPLC测定泮托拉唑钠肠溶胶囊的释放度  

Determination of drug release from Pantoprazole sodium enteric-coated capsule by HPLC

作　　者：刘菲 李宁 顾斌基 刘志山[3] 

机构地区：[1]辽宁省药品检验所,辽宁沈阳110023 [2]沈阳铸造研究所,辽宁沈阳110022 [3]沈阳市卫生监督所,辽宁沈阳110014

出　　处：《华西药学杂志》2007年第3期320-321,共2页West China Journal of Pharmaceutical Sciences

摘　　要：目的建立测定泮托拉唑钠肠溶胶囊释放度的方法。方法采用HPLC法,色谱柱为Kromasil C18（150 mm×4.6 mm,5μm）,流动相为磷酸盐缓冲液-乙腈（60：40）,检测波长289 nm,流速1.0 ml·min^-1。结果线性范围为4.6-31.0μg·ml^-1（r=0.9999）,平均回收率为98.48%,RSD=0.65%（n=9）。结论所用方法简便快速,专属性强,灵敏度高,准确度好。OBJECTIVE To establish an HPLC method for determination of drug release of Pantoprazole sodium enteric - coated cap- sule. METHODS Kromasil C18（150 mm ×4.6 mm,5 μm） colurmn was used. The mobile phase was phosphate buffer- aceotonirtrile（60 ： 40）. The deteetion wavelength was 289 nm. The flow rate was 1.0 ml·in^-1.RESULTS The linear range of Pantoprazole sodium in this method was 4.6 -31.0 μgμ·ml^-1（ r = 0. 9999）. The average recovery was 98.48% ,RSD = 0.65% （n = 9）. CONCLUSION The method could eliminate the interference of the Placebo and blank capsule effectively when the drug release of Pantoprazole sodium enteric -coated capsule was determined. The method is accurate,reliable, rapid and convenient.

关 键 词：泮托拉唑钠 高效液相色谱法 释放度 

分 类 号：R917[医药卫生—药物分析学]