HPLC鉴别西藏黄连和云南黄连中的生物碱  

Content identification of alkaloids in Coptis teeta and C. teetoides by HPLC

作　　者：杨林[1] 陈璐[2] 王天志[1] 杨兵[1] 

机构地区：[1]四川大学华西药学院,四川成都610041 [2]四川生殖卫生学院,四川成都610041

出　　处：《华西药学杂志》2007年第3期344-346,共3页West China Journal of Pharmaceutical Sciences

摘　　要：目的建立鉴别西藏黄连和云南黄连的方法。方法采用HPLC法,色谱柱为Diamonsil C18(150 mm×6 mm,5μm),流动相为乙腈-0.02 mol.L-1KH2PO4溶液(30:70,内含SDS 1.275 g.L-1),流速1 ml.min-1,检测波长268 nm,柱温30℃。结果云南黄连中非洲防己碱、药根碱和巴马汀的含量明显高于西藏黄连,且含少量的表小檗碱,西藏黄连中表小檗碱的含量未达检测限。结论非洲防己碱、药根碱和巴马汀可以作为鉴别西藏黄连与云南黄连的特征性成分。OBJECTIVE To identify Coptis teeta and C. teetoides. METHODS HPLC method was adopted with Diamonsil C18 scolumn（150 mm ×6 mm,5 μm）. The mobile phase consisted of acetonitrile - phosphate （30 ： 70） and the flow rate was 1.0 ml·min^-1. RESULTS The contents of columbamine, jatrorrhizine, palmatine in rhizome of C. teetoides C.Y. Cheng were obviously higher than those in rhizome of C. teeta Wall. C. teetoides which contained a small amount of epiberberine only, and it didn＇ t reach the limit of detection of C. teeta. CONCLUSION Columbamine,jatrorrhizine and palmatine could be considered as the characteristic components for identification of Coptis teeta and C. teetoides.

关 键 词：西藏黄连 生物碱 云南黄连 高效液相色谱 

分 类 号：R917[医药卫生—药物分析学]