指纹图谱研究灯盏细辛中具有视神经保护作用的有效成分  

HPLC-based activity profiling of Erigeron breviscapus for optic neuroprotection

作　　者：张静[1] 张艺[1] 杨文宇[1] 盛艳梅[1] 段俊国[1] 王毓杰[1] 

机构地区：[1]成都中医药大学民族医药研究所,四川成都610075

出　　处：《华西药学杂志》2007年第2期121-123,共3页West China Journal of Pharmaceutical Sciences

基　　金：国家自然科学基金(批准号:30271581)

摘　　要：目的研究灯盏细辛具视神经保护作用的有效组分。方法采用体外视网膜神经节细胞存活的影响实验,并结合HPLC-DAD指纹图谱技术。结果明确了灯盏细辛视神经保护有效组分的指纹图谱。结论灯盏细辛中,以野黄芩苷为主的黄酮类成分和咖啡酰类成分为其视神经保护的有效组分。OBJECTIVE To investigate the optic neuroprotection components of Erigeron breviscapus. METHODS With the aid of HPLC - based profiling techniques, viability of in-vitro retinal ganglion cells cultured with extracts of Erigeron breviscapus was tested. RESULTS HPLC - based activity fingerprint of optic neuroprotective components of E. breviscapus was identified. CONCLUSION Flavonoids and caffeoyl compounds were the active components of E. breviscapus with optic neuroprotective effect.

关 键 词：灯盏细辛 视神经保护 有效组分 指纹图谱 视网膜神经节细胞 

分 类 号：R284[医药卫生—中药学]