HPLC测定民族药岩陀药材中岩白菜素的含量  

Content determination of bergenin in ethnodrug Rodgersia sambucifolia Hemsl. by HPLC

作　　者：陈文文[1] 黄勤挽[1] 兰雁[1] 吴纯洁[1] 

机构地区：[1]成都中医药大学,四川成都610075

出　　处：《华西药学杂志》2007年第2期210-211,共2页West China Journal of Pharmaceutical Sciences

摘　　要：目的建立测定民族药岩陀药材中岩白菜素含量的方法。方法采用HPLC法,Kromasil C18柱(250 mm×4.6 mm,5μm);流动相为甲醇-水(25:75);检测波长275 nm。结果线性范围为0.2556-1.2780μg(r=0.9999),平均回收率为99.59%,RSD=1.85%。结论所建立的方法简便准确、灵敏度高,可用于岩陀药材及其制剂的质量控制。OBJECTIVE To establish a method for content determination of bergenin in ethnodrug Rodgersia sambucifolia Hemsl. by HPLC. METHODS Kromasil C18 column （250 mm× 4.6 mm,5 μm） was used. The mobile phase was composed of methanol - water （25 ： 75 ）. Detection wavelength was 275 nm. RESULTS The linearity was obtained over the range of 0.2556 - 1, 2780 μg （ r = 0. 9999）. The mean recovery was 99.59% with RSD of 1.85%. CONCLUSION The method is sensitive, simple and accurate. It can be used to control quality of Rodgersia sambucifolia Hemsl. and its preparations.

关 键 词：岩陀 高效液相色谱法 岩白菜素 民族药 

分 类 号：R917[医药卫生—药物分析学]