柱色谱-分光光度法测定菝葜中总黄酮的含量  

Determination of total flavones in Smilax China L.from different habitats by column chromatogram-spectrophotometry

作　　者：李文霞[1] 颜彦[2] 陈忠光[2] 从仁怀[2] 叶晓川[2] 杨祥良[2] 

机构地区：[1]华中科技大学化学系,湖北武汉430074 [2]华中科技大学生命科学与技术学院,湖北武汉430074

出　　处：《华西药学杂志》2007年第2期212-214,共3页West China Journal of Pharmaceutical Sciences

摘　　要：目的建立测定菝葜中总黄酮含量的方法。方法测定波长500 nm,以芦丁为对照品,采用柱色谱-分光光度法测定不同产地菝葜中总黄酮的含量。结果芦丁在8.02-48.12μg.m l-1范围内线性关系良好(r=0.9996),平均回收率为96.70%,RSD=1.24%。结论所建方法操作简便、结果准确、重复性好,可用于药材的质量控制。OBJECTIVE To establish a method for the determination of total flavones in Smilax China L. METHODS The content of total flavones in Smilax china L. was determined at 500 nm by column chmmatogram - spectrophotometry with rutin as standard control. RESULTS Rutin showed a good linear curve in the range of 8.02 - 48.12 μg· ml^ - 1 （ r = 0. 9996 ） . The recovery of total flavones was 96.70% with RSD of 1.24%. CONCLUSION The method is simple, rapid and accurate. It can be used for determining the content of total flavones in Smilax China L..

关 键 词：菝葜 总黄酮 含量测定 柱色谱-分光光度法 

分 类 号：R927[医药卫生—药学]