人参再造丸含量测定中样品制备方法的改进  

Improved sample preparation method for content determination of berberine in Renshen Zaizao pills

作　　者：廖红娟 罗勇 张美英 王一飞 

机构地区：[1]广州暨南生物医药研究开发基地,广东广州510632

出　　处：《华西药学杂志》2007年第2期216-217,共2页West China Journal of Pharmaceutical Sciences

基　　金：广东省自然科学基金团队项目(2004E039213);广东省社会发展计划项目(2005B30101010)

摘　　要：目的测定人参再造丸中盐酸小檗碱的含量。方法采取饱和流出式固相萃取技术对样品进行纯化,ZorbaxSB-C18色谱柱(250 mm×4.6 mm,5μm),流动相为乙腈-0.05 mol.L-1磷酸二氢钾溶液(28:72),流速1 ml.min-1,检测波长345nm。结果盐酸小檗碱进样量在0.0436-0.4360μg范围内与峰面积呈良好的线性关系(r=0.9999),平均回收率98.37%,RSD=1.6%(n=6)。结论制备方法能有效去除基质,保护色谱柱,所得样品组分分离度好,简便快速,重复性好,结果准确可靠,可用于制备人参再造丸质量控制所需的供试品溶液。OBJECTIVE To determine berberine in Renshen Zaizao pills by SPE - HPLC. METHODS The sample was purified by saturated flow - through SPE and analysized by HPLC on column of ZorbaxSB - C18 （250 mm × 4.6 mm,5 μm） with mobile phase of acetonitrile - 0.05 mol· L^-1 KH2PO4 （28 ： 72）, flow rate of 1.0 ml·min^ -1 and the detection wavelength at 345 nm. RESULTS The calibration curve showed a good linear correlation within the concentration range of 0. 0436 - 0.4360 μg （ r = 0. 9999）, and the average recovery was 98.37% with the RSD of 1. 6% （n =6） for berberine. CONCLUSION The method can remove ground - substance to protect the column effectively. It is convenient, rapid, reproducible, accurate and reliable with good separation, which can be applied to prepare the sample solution of Renshen Zaizao pills.

关 键 词：人参再造丸 盐酸小檗碱 固相萃取 高效液相色谱法 

分 类 号：R917[医药卫生—药物分析学]