PLA-PAMAM纳米粒作为非病毒基因载体的研究  

PLA-PAMAM nanoparticles: non-viral carriers for gene delivery

作　　者：周林珠[1] 甘璐[1] 杨祥良[1] 

机构地区：[1]华中科技大学生命科学与技术学院,湖北武汉430074

出　　处：《华中科技大学学报（自然科学版）》2007年第6期122-125,共4页Journal of Huazhong University of Science and Technology(Natural Science Edition)

基　　金：国家高技术研究发展计划资助项目(2004AA2Z3212)

摘　　要：采用乳化-分散-蒸发方法制备了表面含有聚酰胺-胺树状大分子的聚乳酸纳米粒,并将DNA与该阳离子纳米粒复合,研究了复合物的理化性质、细胞毒性和转染效率.结果表明在氮/磷比为2以上时,DNA与阳离子纳米粒形成了稳定且带正电的复合物,能保护DNA抵御核酸酶的降解,且复合物的细胞毒性较小.在无血清介质中,阳离子纳米粒可以将DNA有效地转移到细胞内得到表达,转染效率与未纳米化的PAMAM相比有明显的提高,且转染的最佳氮/磷比为2-5.Poly（lactic acid） （PLA） nanoparticles bearing polyamidoamine （PAMAM） dendrimer on their surface were prepared by emulsion-diffusion-evaporation technique and evaluated as gene delivery carriers in this study. Physicochemical properties, cytotoxicity and transfection efficiency of the complexes prepared by DNA and cationic nanoparticles were determined to search for the relationship between structural properties and transfection activity. DNA was shown to form stable and positively charged complexes with cationic nanoparticles at N/P ratios above 2. The complexes were efficient to provide protection against enzymatic cleavage and were low-toxic in HepG2 and NIH-3T3 cells. In serum-free medium, cationic nanoparticles could efficiently transport DNA into the cells and showed higher transfection efficiency than that of PAMAM. Furthermore, the optimum ratios of N to P for transfection are between 2-5.

关 键 词：基因转移载体 聚酰胺-胺树状大分子 聚乳酸 纳米粒 转染 

分 类 号：Q5[生物学—生物化学]