螺旋藻培养条件响应面法优化的研究  

Optimization of Cultivation Conditions for Biomass by Spirulina Platensis Using RSM

作　　者：谯顺彬[1,2] 张奕婷[1,2] 迟海洋[1,2] 张义明[1,3] 

机构地区：[1]贵州省发酵工程与生物制药重点实验室,贵州贵阳550003 [2]贵州大学化学工程学院,贵州贵阳550003 [3]贵州科技工程职业学院,贵州贵阳550008

出　　处：《贵州科技工程职业学院学报》2007年第2期1-7,共7页GUIZHOU SCIENCE AND TECHNOLOGY PROFESSIONAL COLLEGE

基　　金：国家自然科学基金项目（30460039）,教育部“春晖计划”项目,贵州省科技攻关项目（2004GGT022）,贵州省优秀科技教育人才省长专项基金资助项目（黔科教办200304号）.

摘　　要：设计了用于螺旋藻培养的新型气升式光生物反应器，并用响应曲面法对其培养条件进行优化。实验选取影响螺旋藻生长的4个关键因子即光照强度、通气量、培养时间和接种量，并对其最佳水平范围进行研究，建立了以藻体干重为响应值的二次多项式方程。实验结果表明，4个因子对藻体生长的影响大小依次为光照强度、培养时间、装液量、通气量；对方程解逆矩阵可知，当光照强度、通气量、培养时闻和接种量分别达最佳水平44001x、212．2L／h、8．8d和7．2L时，DW最大值为1．277g／L。In this paper, a novel air-lift photo-bioreactor was designed to cultivate the Spirulina platensis, employing the Response surface methodology to optimize the culture conditions of biomass concentration. The four critical factors selected for investigation were light intensity, air flow, time of cultivation and volume of medium. Then research the optimization concentration levels and the relations between these factors and building a quadratic regression equation with DW as the dependent. According to the experiment results, the ordinal relation from strong to inferior influence the growth of SP is light intensity, time of cultivation, volume of medium, air flow. FinaLly, by solving the inverse matrix from the equation, the optimal conditions for obtaining the maximum 1. 277g/L of DW were 44001x, 212.2L/h,8.8d and 7.2 L respectively.

关 键 词：气升式光生物反应器 螺旋藻 响应曲面法 

分 类 号：Q949.22[生物学—植物学]