施磷水平对不同基因型大豆品种硝酸还原酶活性影响  

THE AFFECTION OF PHOSPHOURS LEVEL TO DIFFERENT GENOTYPE SOYBEAN NITRATE REDUCTASE(NR) ACTIVITIES

作　　者：蔡柏岩[1] 葛菁萍[1] 祖伟[2] 

机构地区：[1]黑龙江大学生命科学学院 [2]东北农业大学,哈尔滨150030

出　　处：《大豆科学》2007年第3期359-362,共4页Soybean Science

基　　金：黑龙江省教育厅资助项目(10543074)

摘　　要：选用东农42(高蛋白品种)、合丰25(中间型品种)、东农46(高油品种)3个基因型大豆品种作为试验材料,采用盆栽,在每kg土壤施N和K2O各为0.033g基础上,设P0、P5、P10、P154个P处理(即每kg土壤分别施P2O50、0.033、0.067、0.100g)。采用活体法测定了硝酸还原酶(NR)的活性,结果表明:不同品种不同处理功能叶片NR活性从苗期至成熟期逐渐下降。同一品种不同处理间高蛋白品种和中间型品种P10处理叶片NR活性最高,高油品种P5处理叶片NR活性最高。同一处理不同品种间功能叶片NR活性是高蛋白品种最高,高油品种最低。三个品种中高蛋白品种NR活性最高,说明其氮代谢能力强,有利于提高蛋白质的含量。Dongnong 42 （high protein cultivar）, Hefeng 25 （middle cultivar） and Dongnong 46 （high soil cultivar）, were detected to compare their NR activities. The soybeans were planted in pots and fertilized with 0.033 g of N and K20 per kg soil,at the same time,four levels of phosphorous treatments were designed,which were P0 .P5 .P10 .P15 （i. e. ,0.0. 033.0. 067.0. 100 g of P2 O5per kg soil）. The NR activities was determined with in vitro method. In each cultivar under each treatment,NR activities in functional leaves were gradually decreased from seedling stage to ripening stage,In the same cultivars under each treatment,the highest NR activities in functional leaves were at P10 treatment in high protein variety and middle variety,at P5 treatment in high oil variety. In the same treatment with each species, the NR activities in functional leaves were the highest in high protein variety and the lowest in high oil variety. The highest NR activities in high protein variety showed the best nitrogen metabolic activity and the increase of protein content.

关 键 词：大豆 磷素水平 硝酸还原酶活性 

分 类 号：S565.1[农业科学—作物学]