流式细胞仪检测HLA-B27抗原在强直性脊柱炎诊断中的意义  

Significance of detection of the HLA-B27 expression with flow cytometry in diagnosis of ankylosing spondylitis

作　　者：安新业[1] 刘骙骙[2] 陈佳荣[3] 周玉明[3] 阮月芹[1] 李凤英[1] 

机构地区：[1]滨州医学院附属医院检验科,滨州市256603 [2]滨州医学院护理系 [3]滨州医学院附属医院中心实验室

出　　处：《滨州医学院学报》2007年第3期178-179,共2页Journal of Binzhou Medical University

摘　　要：目的经流式细胞仪(FCM)检测外周血T淋巴细胞表面HLA-B27抗原,探讨其在强直性脊柱炎诊断中的临床意义。方法分别用流式细胞仪和微量淋巴细胞毒法检测外周血T淋巴细胞表面HLA-B27抗原并进行对比。结果流式细胞仪检测86例强直性脊柱炎患者标本,79例阳性,微量淋巴细胞毒法检测为76例阳性。流式细胞仪和微量淋巴细胞毒法检测HLA-B27抗原无显著性差异。结论通过流式细胞仪检测HLA-B27抗原,方法简便,稳定性好,重复性高。Objective To study the clinical evaluation of HLA-B27 by flow cytometry in the diagnosis of ankylosing spondylitis. Methods Micro-lymphocytotoxicity test and flow cytometry were adopted to detect HLA-B27 expression and the following results were compared and analyzed. Results The positive incidence of HLA-B27 with flow cytometry was 91. 86 % （79/86） in ankylosing spondylitis patients , while the positive incidence with micro-lymphocytotoxicity was 88.37% （ 76/86 ）. The difference between the two methods was not significant （P 〉 0. 05 ）. Conclusion The detection of HLA- B27 expression with FCM is an easy , stable , accurate method with high repeatability.

关 键 词：HLA-B27抗原 流式细胞仪 强直性脊柱炎 

分 类 号：R446[医药卫生—诊断学]