PU微胶囊的合成、表征及降解性能研究  

Research on Structure and Property of Polyurethane Microcapsule

作　　者：张志斌[1] 彭雪林[1] 郝静[1] 陈慧清[1] 单连海[1] 

机构地区：[1]西南交通大学生物工程学院,成都610031

出　　处：《世界科技研究与发展》2007年第2期86-89,共4页World Sci-Tech R&D

基　　金：国家自然科学基金项目(50573061);四川省应用基础研究项目(03JY029-059)

摘　　要：本文采用预聚-扩链-中和-分散溶解法，一步合成出聚氨酯（PU）水溶液，再用凝聚相分离法合成出Pu微胶囊。由傅立叶变换红外光谱（FT—IR）研究了PU微胶囊的化学结构；使用差示扫描量热仪（DSC），对PU微胶囊的玻璃化转变及微相分离结构进行了研究；通过扫描电子显微镜（SEM）的观测，对PU微胶囊的表面及剖面形态进行了研究，并测定出PU微胶囊直径约2．5mm，内腔直径50～600μm，膜孔直径为20～120nm；研究了PU微胶囊在磷酸缓冲液（PBS）中的降解行为，发现本实验合成的PU微胶囊基本上是不降解的。这种PU微胶囊有利于转基因CHO细胞的包覆，有利于临床应用中进行腹腔植入。The PU water solutions were synthesized by combining prepolymerization, chain - extension, neutralization and dispersion-diffluenee methods in one step for the first time. And the PU microcapsules were synthesized by coacervation phase separation method. The chemical structure of PU microcapsale was researched by IR （ Infrared Spectrum）. The glass transformation and microphase separation structure of PU microcapsule were researched by DSC（ Differential Scanning Calorimetry）. The morphology structures about surface and section plane of PU microcapsule were researched by SEM（ Scanning Electron Microscope）. We measured that the diameter of the globose PU microcapsule is 2.5 mm or so,the cavity diameter in the PU microcapsule is 50～600 μm and the membrane orifice diameter of PU microcapsule is 20～120 nm. The degradation property was researched in PBS（ phosphate buffer solution）. From the degradation experiment and the curve of weight loss to time of the PU microcapsule we can see that the PU microcapsule is indegradable. So this PU microcapsule makes for enwrapping the transgenic CHO cell and being embedded into renter in the clinical applications. This work is very significant in clinical application.

关 键 词：转基因CHO细胞 凝聚相分离 PU微胶囊 微相分离结构 

分 类 号：TQ460[化学工程—制药化工]